Plant Growth Enhancement of Seeds Immersed in Plasma Activated Water
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Plant Growth Enhancement of Seeds Immersed in Plasma Activated Water Thapanut Sarinont, Ryu Katayama, Yosuke Wada, Kazunori Koga, and Masaharu Shiratani Kyushu University, Motooka 766, Fukuoka 819-0395, Japan. *E-mail: [email protected] ABSTRACT We have produced plasma activated water (PAW) using air, O2, N2, He and Ar atmospheric pressure dielectric barrier discharge plasma irradiation to deionized water. Then, PAW was kept for 1 hour or 1 day at room temperature to reduce concentrations of short lifetime reactive oxygen species and reactive nitrogen species before supplying to plants. O2, air and N2 PAW induces growth enhancement of plants. For 1 hour PAW supply the longest seedling length after 3 days cultivation is 1.62, 1.38, 1.13, 1.12, and 1.04 times long for air, O2, He, N2, and Ar plasmas compared with the length for thecontrol, whereas for 1 day PAW supply it is 1.52, 1.28, 1.13, 1.10, and 1.08 times long for air, O2, He, N2 and Ar. Therefore, long lifetime reactive oxygen nitrogen species in PAW is effective for the growth enhancement. INTRODUCTION Atmospheric-pressure nonthermal plasmas have been actively studied and developed for various applications [1-16]. One of emerging and important applications of such plasmas is agriculture. There are three ways of plasma irradiation: direct plasma irradiation, remote plasma irradiation, and use of plasma activated liquids. Direct plasma irradiation provides the highest flux of reactive oxygen species (ROSs), reactive nitrogen species (RNSs), ions, electrons, and photons, while it sometimes gives damage to living thing. Remote plasma irradiation provides the significant flux of ROSs, RNSs, ions and photons with less damage. Plasma activated liquids provide rather low concentration of short lifetime ROSs and RNSs and high concentration of long lifetime ROSs and RNSs and their reaction products with little damage. Since most living thing contains liquids and often lives in liquids, plasma activated liquids are attractive routes for biological applications such as medicine and agriculture. In the short term, the world is facing the threat of another food crisis similar to what happened in 2007-2008 when prices of commodities like rice spiked [17-21]. In the long term, the world needs to produce at least 50% more food to feed 9 billion people by 2050. One possible solution to the global food crisis is to improve agricultural productivity. Atmospheric pressure nonthermal plasmas can contribute to solve the issue. From this viewpoint, we also have detected chemicals in plasma activated liquids and have developed a method of visualization of ROSs transport in water [22-28]. So far, we have shown that atmospheric pressure dielectric barrier discharge (DBD) plasma irradiation to plant seeds can induce continuous growth enhancement of the plants, shorten the harvesting period, and improve significantly the crop yield [29-36]. ROSs and RNSs produced by plasmas often interacts with living cells surrounded by water. Therefore, interaction among plasmas, wate
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