Plantlet regeneration through indirect shoot organogenesis and somatic embryogenesis in Justicia gendarussa Burm. f., a
- PDF / 926,196 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 69 Downloads / 217 Views
ORIGINAL ARTICLE
Plantlet regeneration through indirect shoot organogenesis and somatic embryogenesis in Justicia gendarussa Burm. f., a medicinal plant N. Bhagya & K. R. Chandrashekar & Anitha Karun & U. Bhavyashree
Received: 21 June 2012 / Accepted: 8 November 2012 # Society for Plant Biochemistry and Biotechnology 2012
Abstract A protocol for the regeneration of a large number of plantlets via indirect shoot organogenesis and somatic embryogenesis has been developed from the stem and leaf explants of Justicia gendarussa Burm. f. The callus was efficiently induced from the explants using Murashige and Skoog (MS) medium supplemented with α-Naphthalene acetic acid (NAA) + Benzyl amino purine (BAP) (1.0+ 0.1 mg/l). The highest number of plantlets through indirect shoot organogenesis was obtained when the callus was subcultured to MS medium with BAP + NAA (0.1+1.0 mg/l). The maximum number of plantlets via somatic embryos was obtained in the medium with BAP + NAA (1.0+0.1 mg/l) for stem derived calli and Kinetin (Kn) + NAA (2.0 + 0.1 mg/l) for leaf derived calli. The in vitro developed shoots were rooted well in half strength MS medium supplemented with 0.5 mg/l of Indole-3-acetic acid (IAA). The in vitro regenerated plantlets were hardened using a mixture of sterile sand:soil:manure (1:1:1). The present study is the first report on the regeneration of plants through somatic embryogenesis from stem and leaf derived calli of J. gendarussa. Keywords Justicia gendarussa . Shoot organogenesis . Somatic embryogenesis . Histology N. Bhagya : K. R. Chandrashekar (*) Department of Applied Botany, Centre of Excellence, Mangalore University, Mangalagangotri, Mangalore 574199, Karnataka, India e-mail: [email protected] A. Karun : U. Bhavyashree Biotechnology Section, CPCRI, Kasaragod 671124, Kerala, India
Abbreviations BAP Benzyl amino purine IAA Indole 3 acetic acid Kn Kinetin MS Murashige and Skoog NAA Naphthalene acetic acid TDZ Thidiazuron (N-phenyl-N’-1-thiadiazol-5-ylurea) Zea Zeatin
Introduction The plant regeneration through in vitro culture technique involves the exposure of the explants to various growth regulators, vitamins, amino acid conditions that activates a new developmental pathway, either through direct organogenesis or through somatic embryogenesis in Pisum sativum (Zhihui et al. 2009). The regeneration of plantlets through indirect shoot organogenesis was reported in Ocimum gratissimum L. (Gopi et al. 2006), Tinospora cordifolia (Raghu et al. 2007), Solanum nigrum (Linn.) (Shridhar and Naidu 2011) and through somatic embryogenesis in Dalbergia sissoo (Singh and Chand 2003), Bacopa monnieri (Jain et al. 2010) and Pulsatilla koreana Nakai (Lin et al. 2011) were reported. In vitro regeneration of plants through somatic embryogenesis is preferred over other methods as the embryos have singlecell origin, low frequency of chimeras and the production of a high number of regenerates (Ammirato 1983). Justicia gendarussa Burm. f. commonly known as Aduthoda gida, Karalakki gida in Kannada is well known for its an
Data Loading...
