Potential for Osteogenic and Chondrogenic Differentiation of MSC
The introduction of mesenchymal stem cells (MSC) into the field of tissue engineering for bone and cartilage repair is a promising development, since these cells can be expanded ex vivo to clinically relevant numbers and, after expansion, retain their abi
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Potential for Osteogenic and Chondrogenic Differentiation of MSC Antonina Lavrentieva, Tim Hatlapatka, Anne Neumann, Birgit Weyand and Cornelia Kasper Abstract The introduction of mesenchymal stem cells (MSC) into the field of tissue engineering for bone and cartilage repair is a promising development, since these cells can be expanded ex vivo to clinically relevant numbers and, after expansion, retain their ability to differentiate into different cell lineages. Mesenchymal stem cells isolated from various tissues have been intensively studied and characterized by many research groups. To obtain functionally active differentiated tissue, tissue engineered constructs are cultivated in vitro statically or dynamically in bioreactors under controlled conditions. These conditions include special cell culture media, addition of signalling molecules, various physical and chemical factors and the application of different mechanical stimuli. Oxygen concentration in the culture environment is also a significant factor which influences MSC proliferation, stemness and differentiation capacity. Knowledge of the different aspects which affect MSC differentiation in vivo and in vitro will help researchers to achieve directed cell fate without the addition of differentiation agents in concentrations above the physiological range.
Antonina Lavrentieva and Tim Hatlapatka contributed equally. A. Lavrentieva (&) Institut für Technische Chemie, Leibniz Universität Hannover, Callinstrasse 5, 30167 Hannover, Germany e-mail: [email protected] T. Hatlapatka A. Neumann C. Kasper Department für Biotechnologie, Universität für Bodenkultur, Muthgasse 18, 1190 Wien, Austria e-mail: [email protected] A. Neumann e-mail: [email protected] C. Kasper e-mail: [email protected] B. Weyand Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg Strasse 1, 30625 Hannover, Germany e-mail: [email protected]
A. Lavrentieva et al.
Keywords Chondrogenesis Hypoxia Mesenchymal stem cells Mesenchymal stromal cells Mechanical stimulation Osteogenesis
Abbreviations ALP AD b-FGF BM BMPs ESC GAG GvHD h HA HLA HUCPVC ISCT LIPUS LLLI MAPK MMPs MSC PBL PG PLGA PFF r TGF-b TE UC UCB WJ 3D
Alkaline phosphatase Adipose-derived Basic fibroblast growth factor Bone marrow Bone morphogenic proteins Embryonic stem cell Glycosaminoglycan Graft-versus-host disease Human Hyaluronic acid Human leukocyte antigen Human umbilical cord perivascular cells International Society for Cellular Therapy Low-intensity pulsed ultrasound Low-level light irradiation Mitogen-activated protein kinases Matrix metalloproteases Mesenchymal stromal cell Peripheral blood lymphocytes Proteoglycan Poly(L-lactide-co-glycolide) Pulsating fluid flow Rabbit Transforming growth factor-beta Tissue engineering Umbilical cord Umbilical cord blood Wharton’s jelly Three-dimensional
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Introduction.............................................................................................
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