Protective Impact of Edaravone Against ZnO NPs-induced Oxidative Stress in the Human Neuroblastoma SH-SY5Y Cell Line
- PDF / 5,557,155 Bytes
- 22 Pages / 595.276 x 790.866 pts Page_size
- 30 Downloads / 188 Views
ORIGINAL RESEARCH
Protective Impact of Edaravone Against ZnO NPs‑induced Oxidative Stress in the Human Neuroblastoma SH‑SY5Y Cell Line Sanjiv Singh1,3 · Upendr Gautam2 · F. V. Manvi3 Received: 19 January 2020 / Accepted: 13 November 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Extensive applications of ZnO NPs (zinc oxide nanoparticles) in daily life have created concern about their biotoxicity. Zinc oxide nanoparticles induce oxidative stress, inflammation, and apoptosis in neurons. Edaravone applies antioxidant agent and anti-inflammatory impacts in the different cells, as evaluated in both in vitro and in vivo experimental models. This study is designed to explore, how edaravone would avert mitochondrial impairment in human neuronal cells against ZnO NPs-induced toxicity. Accordingly, we analyzed here whether a pretreatment (for 24 h) with edaravone (10–100 μM) would enhance mitochondrial protection in the human neuroblastoma cells SH-SY5Y against ZnO NPs-induced toxicity. We found that edaravone at 25 μM averted the ZnO NPs-induced decrease in the amounts of adenosine triphosphate (ATP), just as on the activity of the complexes I and V. Also, edaravone induced an antioxidant activity by diminishing the levels of lipid peroxidation, protein carbonylation, and protein nitration in the mitochondrial membranes. Edaravone blocked the ZnO NPs-induced transcription factor nuclear factor-κB (NF-κB) upregulation. The inhibition of the heme oxygenase-1 (HO−1) enzyme by zinc protoporphyrin IX (ZnPP IX, 10 μM) smothered the preventive impacts brought about by edaravone with respect to mitochondrial function and inflammation. After this examination, it can be concluded that edaravone caused cytoprotective impacts in an HO−1-dependent manner in SH-SY5Y cells against ZnO NPs-induced toxicity. Keywords Edaravone · ZnO nanoparticles · Neurotoxicity · Heme oxygenase pathway · Neuroprotective effect
Introduction Among different metal oxides, zinc oxide nanoparticles (ZnO NPs) are the most generally utilized NPs for their one of a kind physical and enzymic properties and simple synthesis (Colvin 2003). ZnO NPs are utilized in an assortment of items, for example, dyes, toothpaste, cosmetics, sunscreens, textiles, wall paints, and other building textiles (Memarzadeh et al. 2015; Osmond and McCall 2010). Moreover, ZnO NPs are utilized in the food industry as added substances * Sanjiv Singh [email protected] 1
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Export Promotions Industrial Park (EPIP), Industrial Area, Hajipur, Bihar, India
2
Vinayaka Mission’s Research Foundation, Ariyanur, Tamil Nadu, India
3
K.L.E. Academy of Higher Education & Research, Belagavi, Karnataka, India
inferable from their antimicrobial potential (Jin et al. 2009), in farming as antifungal (He et al. 2011) and therapeutically as anticancer (Rasmussen et al. 2010). Consequently, the oral introduction to ZnO NPs is expanded alarmingly. A few
Data Loading...
