Pseudomonas citronellolis strain SLP6 enhances the phytoremediation efficiency of Helianthus annuus in copper contaminat
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Pseudomonas citronellolis strain SLP6 enhances the phytoremediation efficiency of Helianthus annuus in copper contaminated soils under salinity stress Sivagnanam Silambarasan & Peter Logeswari & Alexander Valentine & Pablo Cornejo & Velu Rajesh Kannan Received: 19 June 2020 / Accepted: 4 October 2020 # Springer Nature Switzerland AG 2020
Abstract Background and aims Plant growth-promoting bacteria (PGPB)-assisted phytoremediation, is a promising technique for the removal of copper (Cu) from contaminated soils. Moreover, soil salinity is considered to be one of the major threats to plant growth, and may therefore affect the progress and impact of the plant-driven phytoremediation. In this regard, the multiple interactions among Cu-salinity-plant-microbe, remain largely Responsible Editor: Juan Barcelo Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11104-020-04734-7) contains supplementary material, which is available to authorized users. S. Silambarasan (*) : P. Logeswari : P. Cornejo Centro de Investigación en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile e-mail: [email protected] A. Valentine Botany and Zoology Department, Faculty of Science, University of Stellenbosch, Stellenbosch, South Africa P. Cornejo (*) Scientific and Technological Bioresource Nucleus, BIOREN-UFRO, Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile e-mail: [email protected] V. R. Kannan Rhizosphere Biology Laboratory, Department of Microbiology, Bharathidasan University, Tiruchirappalli 620 024 Tamil Nadu, India
unknown. This study aimed to investigate the potential of PGPB on phytostabilization of Cu contaminated soils using Helianthus annuus growing under salinity stress. Methods A pot experiment was carried out to determine the effects of Cu and salinity tolerant PGPB inoculation in the phytoremediation of Cu-spiked soils by H. annuus under salt-stressed and non-stressed conditions. Results Cu- and salinity-tolerant bacterial strains were isolated from Cu contaminated soils. Out of the eight bacterial isolates, SLP6 was selected and identified as Pseudomonas citronellolis by 16S rRNA and characterized in terms of its Cu resistance (up to 400 mg L−1), salinity tolerance (up to 8% NaCl, w/v) and plant growth promoting (PGP) traits. Strain SLP6 displayed 1aminocyclopropane-1-carboxylic acid deaminase activity, indole-3-acetic acid production, siderophores production, and phosphate solubilization under normal, Cu (100–400 mg L−1) and NaCl (2–8%, w/v) stressed conditions. The inoculation of H. annuus seeds with SLP6 strain significantly enhanced the plant growth, chlorophyll content, anti-oxidant enzymes production and Cu accumulation potential under Cu contamination with and without salt stress, whereas they reduced lipid peroxidation. Conclusions Pseudomonas c
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