Pulse Field Gel Electrophoresis Methods and Protocols
This volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the h
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Kieran Jordan Marion Dalmasso Editors
Pulse Field Gel Electrophoresis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series editor John M.Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Pulse Field Gel Electrophoresis Methods and Protocols Edited by
Kieran Jordan Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork, Ireland
Marion Dalmasso Department of Microbiology & Alimentary Pharmabiotic Centre, University College Cork, Co. Cork, Ireland
Editors Kieran Jordan Teagasc Food Research Center, Moorepark Fermoy, Co. Cork, Ireland
Marion Dalmasso Department of Microbiology & Alimentary Pharmabiotic Centre University College Cork Co. Cork, Ireland
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2598-8 ISBN 978-1-4939-2599-5 (eBook) DOI 10.1007/978-1-4939-2599-5 Library of Congress Control Number: 2015935078 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface First described in 1984 for the separation of large DNA fragments, pulsed field gel electrophoresis (PFGE) has emerged as the “gold standard” method for molecular subtyping of bacteria. Comparative analysis of different isolates of the same bacterial species is important for pathogenic and nonpathogenic bacteria. For pathogenic bacteria, PFGE has revolutionized epidemiological investigations for disease surveillance studies and contributed significantly to disease monitoring and control programs, facilitating the tracking of infection and contamination. For nonpathogenic bacteria, PFGE can be used for monitoring survival of particular strains or tracking contamination routes, among
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