Quality Assessment of Medicinal Leaves Through Biospeckle Technique
In this paper, we report investigations undertaken towards visualization of microbiological objects in medicinal leaves using biospeckle activity approach. Both fresh and infected samples of Ficus Religiosa (peepal leaf) are illuminated by a laser source
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Abstract In this paper, we report investigations undertaken towards visualization of microbiological objects in medicinal leaves using biospeckle activity approach. Both fresh and infected samples of Ficus Religiosa (peepal leaf) are illuminated by a laser source for generation of biospeckle pattern. Dynamic variation in activity images were grabbed by a Charge Couple Device (CCD) camera and quantized into 8 bit digital image using frame grabber. For image analysis revised version of conventional Fujii’s method i.e. weighted parameterized Fujii method is used. It shows high contrast as compared to the other existing methods when sample is illuminated uniformly. The current study aims to distinguish between fresh and infected leaves for improvement of quality of Ayurvedic medicines.
1 Introduction Leaves of medicinal plants have a great importance in Indian Ayurvedic pharmaceutical industry [1]. Mainly, juice and poultice of leaves are used for healing various diseases. Under certain environmental conditions, it can be affected by several microbiological organisms (fungi, bacteria, viruses, etc.) which not only reduce its medicinal attributes but also incorporate some harmful effects on human body when consumed. One of the most prevailing fungal plant syndromes is leaf spotting. Leaf spot syndromes are produced by many different types of fungi like
J. Dhanotia V. Bhatia Signals and Software Group, Discipline of Electrical Engineering, Indian Institute of Technology Indore, Simrol, Madhya Pradesh 453446, India L. Bopche S. Prakash (&) Photonics Laboratory, Institute of Engineering & Technology, Devi Ahilya University, Indore, Madhya Pradesh 452001, India e-mail: [email protected] © Springer Nature Singapore Pte Ltd. 2017 I. Bhattacharya et al. (eds.), Advances in Optical Science and Engineering, Springer Proceedings in Physics 194, DOI 10.1007/978-981-10-3908-9_47
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Marssonia, Septoria, etc. These comprise a large group of fungi, the affiliates of which are responsible for different diseases in human body. Powdery Mildew is another type of disease present in a leaf, for the plants raised in dry climates. General symptoms include a white or grayish powdery growth on leaves. These are also responsible for the occurrence of diseases in human body. The biospeckle technique is an optical phenomenon based on scattering, when a biological sample is illuminated by laser light [2]. This scattered light interferes and generates a granular patterns consisting of random dark and bright areas on the observation plane. The spatial aberrance of speckle pattern is determined by using the characteristic of rough surface. When the phenomenon is used to observe biological samples, such type of pattern is called as biospeckle. This phenomenon has been successfully used for the measurement of blood flow [3], activity and quality evaluation of fruits [4, 5], assessment of seed viability [6], drying process of non-biological sample (e.g. paint, fevicol) [7, 8], bruising in fruits [9], detection
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