Recombinant Proteins from Plants Methods and Protocols
This volume provides up-to-date scientific achievements from the world’s top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various a
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Jacqueline MacDonald Igor Kolotilin Rima Menassa Editors
Recombinant Proteins from Plants Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Recombinant Proteins from Plants Methods and Protocols Second Edition
Edited by
Jacqueline MacDonald London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada
Igor Kolotilin Department of Biology, Western University, London, ON, Canada
Rima Menassa London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada
Editors Jacqueline MacDonald London Research and Development Centre Agriculture and Agri-Food Canada London, ON, Canada
Igor Kolotilin Department of Biology Western University London, ON, Canada
Rima Menassa London Research and Development Centre Agriculture and Agri-Food Canada London, ON, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3288-7 ISBN 978-1-4939-3289-4 (eBook) DOI 10.1007/978-1-4939-3289-4 Library of Congress Control Number: 2015955577 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2009, 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Recombinant protein production in plants is becoming an increasingly attractive alternative to conventional production platforms due to promising cost and safety benefits. Up-to-date scientific achievements from the world’s top researchers are conveniently arranged in the new addition of Recombinant Proteins from Plants as a collection of protocols for use with a variety of plant expression systems. Various aspects of production are cov
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