Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes
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RESEARCH
Open Access
Release kinetics of the model protein FITCBSA from different polymer-coated bovine bone substitutes Julian Lommen1, Lara Schorn1* , Alexis Landers2, Henrik Holtmann3, Karin Berr1, Norbert R. Kübler1, Christoph Sproll1, Majeed Rana1 and Rita Depprich1
Abstract Background: Controlled release of proteins bound to conventional bone substitutes is still insufficient. Therefore, this study evaluates in-vitro release kinetics of the model protein FITC-BSA (fluorescein conjugated bovine serum albumine) from insoluble bovine collagenous bone matrices (ICBM) with different polymer coatings. Analyzes aim at comparing FITC-BSA release from uncoated versus coated ICBM over time to find bone substitute coatings with consistent release profiles. Methods: Release kinetics of FITC-BSA from uncoated as well as coated ICBM with five different polymers (RESOMER R 203 H, RG 503 H, RG 504 H, RG 505, L 206 S) were measured over a period of 11 days (d). Measurements were conducted after 6 h (h), 12 h, 24 h, 3 d, 5 d, 7 d, 9 d and 11 d with six samples for each coated ICBM. Two groups were formed (1) with and (2) without medium change at times of measurement. For each group ANOVA with post-hoc Bonferroni testing was used. Scanning electron microscopy assessed morphologic differences between ICBM coating. Results: In group 1 approx. 70% of FITC-BSA release from uncoated ICBM occurred after 6 h compared to approx. 50% in group 2. Only polymers with medium inherent viscosity, i.e. RESOMER RG 503 H, constantly showed significantly more FITC-BSA release throughout 11 d than uncoated ICBM (p = 0.007). The same was found for group 2 (p = 0.005). No significant differences between PLA and PLGA polymers were found. Scanning electron microscopy results indicate a weak adhesion of polymer coatings to ICBM explaining its rather weak retentive effect on overall FITC-BSA release. Conclusions: Medium molecular size polymers reduce the overall released FITC-BSA from ICBM over time. In clinical practice these polymers may prove ideal for bone substitute materials. Keywords: FITC-BSA, Polymer-coated, Collagen matrices, Bovine, Release kinetics
Background Reconstructive surgery of osseous maxillo-facial structures often requires autologous or allogeneic bone replacement. Due to its osteogenetic, osteoinductive and osteoconductive properties autologous bone is still considered the gold standard bone graft. Disadvantages of autologous bone harvesting are its limited availability and under certain circumstances considerable donor-site * Correspondence: [email protected] 1 Department of Oral and Maxillofacial Surgery, Heinrich-Heine-University, Moorenstraße 5, 40225 Düsseldorf, Germany Full list of author information is available at the end of the article
morbidities perhaps with irreversible damage to patients [1]. Hence, conventional natural or synthetic bone substitute biomaterials have been implemented as volumestable placeholders to enable in-growth of new endogenous bone tissue. In that regard, they promote cell adhesion processe
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