Sequence identification and expression profile of seven Dermacentor marginatus glutathione S-transferase genes
- PDF / 2,448,759 Bytes
- 14 Pages / 439.37 x 666.142 pts Page_size
- 93 Downloads / 188 Views
Sequence identification and expression profile of seven Dermacentor marginatus glutathione S-transferase genes Ercha Hu1,2 · Ying Ma2 · Yunwei Hao2 · Min Li2 · Zhengxiang Hu3 · Ruiqi Song1,2 · Liting Wei2 · Shilong Fan2 · Songqin Chen2 · Xinli Fan2 · Xuejie Zhai2 · Qingyong Guo2 · Chahan Bayin2 Received: 27 July 2020 / Accepted: 15 September 2020 © Springer Nature Switzerland AG 2020
Abstract Dermacentor marginatus is a widespread tick species and a vector of many pathogens in Eurasia. Due to the medical importance of D. marginatus, control measures are needed for this tick species. Currently tick control approaches rely mostly on acaricide application, whereas wrong and irrational acaricide use may result in drug resistance and residue problems. Vaccination as an alternative approach for tick control has been proven to be effective towards some tick species. However, immunization against D. marginatus has not yet reached satisfactory protection. The effort of in silico based analysis could predict antigenicity and identify candidates for anti-tick vaccine development. We carried out an in silico analysis of D. marginatus glutathione S-transferases (DmGSTs) in order to identify blood-feeding induced GSTs as antigens that can be used in anti-tick vaccine development. Phylogenetic analysis, linear B-cell epitope prediction, homology modeling, and conformational B-cell epitope mapping on the GST models were performed to identify highly antigenic DmGSTs. Relative gene expressions of the seven GSTs were profiled through real-time quantitative PCR (RT-qPCR) to outline GSTs up-regulated during blood feeding. The phylogenetic analysis indicated that the seven GSTs belonged to four classes of GST, including one in epsilon-class, one in zeta-class, one in omega-class, and four in mu-class. Linear B-cell epitope prediction revealed mu-class GSTs share similar conserved antigenic regions. The conformational B-cell epitope mapped on the homology model of the GSTs displayed that GSTs of mu-class showed stronger antigenicity than that of other classes. RT-qPCR revealed DmGSTM1 and DmGSTM2 were positively related to blood feeding. In sum, the data suggest that DmGSTM1 and DmGSTM2 could be tested for potential antitick vaccine trials. Keywords Dermacentor marginatus · Glutathione S-transferase · B-cell epitope mapping · Gene expression Electronic supplementary material The online version of this article (https://doi.org/10.1007/s1049 3-020-00546-7) contains supplementary material, which is available to authorized users. * Qingyong Guo [email protected] * Chahan Bayin [email protected] Extended author information available on the last page of the article
13
Vol.:(0123456789)
Experimental and Applied Acarology
Introduction Ticks are obligate hematophagous ectoparasites of great medical and veterinary importance (de la Fuente 2018). As one of the hard ticks adapted to colder regions, Dermacentor marginatus (Sulzer) is a widespread tick species over northern Eurasian (Zhang et al. 2019), which carries and transmits a variety
Data Loading...
