Synthesis of a molecularly imprinted polymer using MOF-74(Ni) as matrix for selective recognition of lysozyme
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RESEARCH PAPER
Synthesis of a molecularly imprinted polymer using MOF-74(Ni) as matrix for selective recognition of lysozyme Jie Li 1 & Miaoxia Ma 1 & Chenhui Zhang 1 & Rui Lu 1 & Lingyi Zhang 1 & Weibing Zhang 1 Received: 1 June 2020 / Revised: 14 July 2020 / Accepted: 30 July 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A molecularly imprinted polymer and metal organic framework were combined to prepare protein imprinted material. MOF74(Ni) was used as a matrix to prepare surface-imprinted material with lysozyme as a template and polydopamine as an imprinting polymer. MOF-74(Ni) not only provides a large surface area (150.0 m2/g) to modify the polymer layer with more recognition sites (Wt (Ni) = 42.24%), but also facilitates the immobilization of lysozyme by using the chelation between Ni2+ of the MOF-74(Ni) and protein. The thin polydopamine layer (10 nm) of the molecularly imprinted material (named MOF@PDAMIP) enables surface imprinting. Benefiting from the thin polymer layer, MOF@PDA-MIP reached adsorption equilibrium within 10 min. The maximum adsorption capacity reaches 313.5 mg/g with the highest imprinting factor (IF) of 7.8. The specific recognition sites can distinguish target lysozyme from other proteins such as egg albumin (OVA), bovine serum albumin (BSA) and ribonuclease A (RNase A). The material was successfully applied to separation of lysozyme from egg white. Keywords Molecularly imprinted polymer . Metal organic framework . Surface imprinting . Lysozyme . Polydopamine
Introduction Molecularly imprinted technology (MIT) is an excellent tool for highly effective separation through polymerization of proper functional monomers and cross-linking agents in the presence of templates [1, 2]. After elution treatment, tailormade stereo cavities which can specifically match with target molecules are obtained [3, 4]. Recently, molecularly imprinted polymers (MIPs) have been widely used in many areas such as chemical sensors [5, 6], solid phase extraction [7], immunoassays [8], and drug delivery [9]. Although sophisticated methods have been developed to obtain MIPs with excellent selectivity for small molecules [10, 11], the technology of imprinting macro-biomolecules, such as proteins or peptides, remains challenging [12, 13]. The main problems include the Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02855-7) contains supplementary material, which is available to authorized users. * Lingyi Zhang [email protected] 1
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
huge size and complex structure of macro-molecules, restricted transfer and elution [14]. To resolve these problems, several methods have been developed over the past decade, including bulk imprinting [15], surface imprinting [16–18] and epitope imprinting [19]. Among these, core-shell MIPs are widely used in surface imprint
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