TaNAC35 acts as a negative regulator for leaf rust resistance in a compatible interaction between common wheat and Pucci
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ORIGINAL ARTICLE
TaNAC35 acts as a negative regulator for leaf rust resistance in a compatible interaction between common wheat and Puccinia triticina Na Zhang1 · Shengliang Yuan1 · Chenguang Zhao1 · Robert F. Park2 · Xiaolei Wen3 · Wenxiang Yang1 · Na Zhang1 · Daqun Liu1 Received: 25 June 2020 / Accepted: 10 November 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract NAC (NAM, AFAT1/2, and CUC2) transcription factors play important roles in plant growth and in resistance to abiotic and biotic stresses. Here, we show that the TaNAC35 gene negatively regulates leaf rust resistance in the wheat line Thatcher + Lr14b (TcLr14b) when challenged with a virulent isolate of Puccinia triticina (Pt). The TaNAC35 gene was cloned from this line, and blastp results showed that its open reading frame (ORF) was 96.16% identical to the NAC35-like sequence reported from Aegilops tauschii, and that it encoded a protein with 387 amino acids (aa) including a conserved NAM domain with 145 aa at the N-terminal alongside the transcriptional activation domain with 220 aa in the C-terminal. Yeast-one-hybrid analysis proved that the C-terminal of the TaNAC35 protein was responsible for transcriptional activation. A 250-bp fragment from the 3′-end of this target gene was introduced to a BSMV-VIGS vector and used to infect the wheat line Thatcher + Lr14b (TcLr14b). The BSMV-VIGS/TaNAC35-infected plant material showed enhanced resistance (infection type “1”) to Pt pathotype THTT, which was fully virulent (infection type “4”) on BSMV-VIGS only infected TcLr14b plants. Histological studies showed that inhibition of TaNAC35 reduced the formation of haustorial mother cells (HMC) and mycelial growth, implying that the TaNAC35 gene plays a negative role in the response of TcLr14b to Pt pathotype THTT. These results provide molecular insight into the interaction between Pt and its wheat host, and identify a potential target for engineering resistance in wheat to this damaging pathogen. Keywords Puccinia triticina · Leaf rust disease · Transcription factors · Virus-induced gene silencing · Negative regulator
Introduction
Communicated by Stefan Hohmann. * Na Zhang [email protected] * Daqun Liu [email protected] 1
Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Hebei Agricultural University, 289 Lingyusi Street, Baoding 071001, Hebei, China
2
Plant Breeding Institute, The University of Sydney, New South Wales 2006, Australia
3
Hebei Normal University of Science & Technology, Qinhuangdao 066000, Hebei, China
Wheat leaf rust is one of the most significant diseases affecting wheat production worldwide. Breeding for dominant resistance to Puccinia triticina (Pt) is the most economical and effective way to protect wheat against this pathogen. However, overreliance on single leaf rust resistance (Lr) genes exerts evolutionary selection pressure on populations of this pathogen, leading to the emergence of races with matching virulence (Park et al. 2002). Plants
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