The circular RNAs differential expression profiles in the metastasis of salivary adenoid cystic carcinoma cells
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The circular RNAs differential expression profiles in the metastasis of salivary adenoid cystic carcinoma cells Rui Ju1,2 · Yanling Huang1,2 · Zeyou Guo1,2 · Lu Han1,2 · Suhui Ji2 · Luyang Zhao2 · Jie Long1,2 Received: 19 July 2020 / Accepted: 16 November 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In order to reveal circular RNAs (circRNAs) differential expression profiles and investigate the function and mechanism of circRNAs in the metastasis of salivary adenoid cystic carcinoma (SACC), microarray was used to detect differentially expressed circRNAs in SACC-83 and SACC-lung metastasis (LM) cell lines. Up-regulated circRNAs were analyzed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses to further predict their function. Expression of candidate circRNA and microRNA (miRNA) was determined using quantitative real-time polymerase chain reaction (qRT-PCR). Constructed circRNA–miRNA–mRNA co-expression network was based on TargetScan, miRanda databases. Wound healing and transwell assays were completed to examine the effects of hsa_circRNA_001982 and miR-181a-5p on cell migration and invasion. qRT-PCR confirmed hsa_circRNA_092556, hsa_circRNA_101379, and hsa_circRNA_001982 up-regulation in SACC-LM. miR-181a-5p was down-regulated in SACC-LM and correlated with upregulated hsa_circRNA_001982. Wound healing and transwell assays indicated that silencing hsa_circRNA_001982 inhibited the migration and invasion of the SACC-LM cells. Furthermore, over-expression of hsa_circRNA_001982 promoted the migration and invasion of SACC-83 cells. Interestingly, up-regulation or down-regulation of miR-181a-5p led to the opposite result in wound healing and transwell assays. Overall, differential expression circRNA profiles in SACC-83 and SACC-LM cells may reveal potential targets and a novel mechanism of circRNAs in the metastasis of SACC. Moreover, the interaction of hsa_circRNA_001982/miR-181a-5p is closely related to the metastasis of SACC cells. Keywords circRNA · miRNA · SACC · Expression profiles Abbreviations circRNA Circular RNA ncRNA Non-coding RNA SACC Salivary adenoid cystic carcinoma LM Lung metastasis Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11010-020-03989-z) contains supplementary material, which is available to authorized users. Rui Ju, Yanling Huang and Zeyou Guo equally contributed to this work.
GO Gene Ontology KEGG Kyoto Encyclopedia of Genes and Genomes miRNA MicroRNA qRT-PCR Quantitative real-time polymerase chain reaction FBS Fetal bovine serum cDNA Complementary DNA siRNAs Small interfering RNAs PBS Phosphate-buffered saline SD Standard deviation MREs MiRNA response elements
* Jie Long [email protected]
Introduction
1
Salivary adenoid cystic carcinoma (SACC) accounts for 25% of major salivary gland malignancies and 50% of minor gland malignancies [1, 2]. SACC is characterized by poor long-term prognosis due to frequent local recurrences
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