The SoNAP gene from sugarcane ( Saccharum officinarum ) encodes a senescence-associated NAC transcription factor involve
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REGULAR PAPER – PHYSIOLOGY/BIOCHEMISTRY/MOLECULAR AND CELLULAR BIOLOGY
The SoNAP gene from sugarcane (Saccharum officinarum) encodes a senescence‑associated NAC transcription factor involved in response to osmotic and salt stress Evelyn A. Carrillo‑Bermejo1 · Samuel David Gamboa‑Tuz1 · Alejandro Pereira‑Santana3,4 · Miguel A. Keb‑Llanes1 · Enrique Castaño2 · Luis Joel Figueroa‑Yañez3 · Luis C. Rodriguez‑Zapata1 Received: 20 April 2020 / Accepted: 8 October 2020 © The Botanical Society of Japan 2020
Abstract Climate change has caused serious problems related to the productivity of agricultural crops directly affecting human wellbeing. Plants have evolved to produce molecular mechanisms in response to environmental stresses, such as transcription factors (TFs), to cope with abiotic stress. The NAC proteins constitute a plant-specific family of TFs involved in plant development processes and tolerance to biotic and abiotic stress. Sugarcane is a perennial grass that accumulates a large amount of sucrose and is a crucial agro-industry crop in tropical regions. Our previous transcriptome analyses on sugarcane that were exposed to drought conditions revealed significant increases in the expression of several NAC TFs through all of the timepoint stress conditions. In this work, we characterize all previously detected sugarcane NAC genes, utilizing phylogenetics and expression analyses. Furthermore, we characterized a sugarcane NAC gene orthologous to the senescence-associated genes AtNAP and OsNAP via transient expression in tobacco calluses, from Arabidopsis and rice respectively, thus we named it the SoNAP gene. Transient localization assays on onion epidermal cells confirmed the nuclear localization of the SoNAP. Expression analysis showed that the SoNAP gene was induced by high salinity, drought, and abscisic acid treatments. The overexpression of the SoNAP gene in tobacco calluses caused a senescence associated phenotype. Overall, our results indicated that the SoNAP gene from sugarcane is transcriptionally induced under abiotic stress conditions and conserved the predicted senescence-associated functions when it was overexpressed in a heterologous plant model. This work provides key insights about the senescence mechanisms related to abiotic stress and it provides a benchmark for future work on the improvement of this economically important crop. Keywords Abiotic stress · Drought · NAC · Phylogenetic analysis · Salinity · Sugarcane Evelyn A. Carrillo-Bermejo and Samuel David Gamboa-Tuz contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10265-020-01230-y) contains supplementary material, which is available to authorized users. * Luis Joel Figueroa‑Yañez [email protected] * Luis C. Rodriguez‑Zapata [email protected] 1
Biotechnology Unit, Centro de Investigacion Cientifica de Yucatan, 97205 Mérida, Yucatan, Mexico
Plant Biochemistry and Molecular Biology Unit, Centro de Investigacion Cientifica de Yucatan, 97205 Mérida, Yu
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