Two-dimensional proteomic analysis of honeybee, Apis mellifera , winter worker hemolymph
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Original article
Two-dimensional proteomic analysis of honeybee, Apis mellifera, winter worker hemolymph Tomas ERBAN1 , Petr L. JEDELSKY2,3,4 , Dalibor TITERA5 1
Department of Pest Control of Stored Products and Food Safety, Laboratory of Proteomics, Crop Research Institute, Drnovska 507/73, Prague 6-Ruzyne, CZ16106, Czech Republic 2 Department of Parasitology, Faculty of Science, Charles University in Prague, Prague, Czech Republic 3 Laboratory of Mass Spectrometry, Faculty of Science, Charles University in Prague, Prague, Czech Republic 4 Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic 5 Bee Research Institute at Dol, Libcice nad Vltavou, Czech Republic Received 11 September 2012 – Revised 7 December 2012 – Accepted 18 December 2012
Abstract – Honeybee (Apis mellifera Linnaeus) colonies in temperate zones produce either summer bees, which have a lifespan of 15 to 48 days, or winter bees, which emerge in late summer and live up to 8 months. Winter bees develop unique physiological conditions characterized by changes in protein composition that appear to be major determinants of honeybee lifespan. We analyzed winter honeybee worker hemolymph using a proteomic approach for the first time. Hemolymph collected from the dorsal vessel of winter honeybees using a glass capillary tube was analyzed using two-dimensional gel electrophoresis followed by MALDI TOF/TOF protein identification. Overall, 93 spots were assigned significance (P
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