Ultrastructure and Molecular Toxicological Effects of the Coronate Scyphomedusa Linuche unguiculata Venom on Giardia duo
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ORIGINAL ARTICLE
Ultrastructure and Molecular Toxicological Effects of the Coronate Scyphomedusa Linuche unguiculata Venom on Giardia duodenalis Juan Luis Morales-Landa 1
&
Fernando Lazcano-Pérez 2 & Roberto Cedillo-Rivera 3 & Judith Sánchez-Rodríguez 2
Received: 18 June 2020 / Accepted: 4 November 2020 # Institute of Molecular Biology, Slovak Academy of Sciences 2020
Abstract Cnidarian venoms are rising as a new source of potential pharmacologically active molecules. They have been investigated for their neurotoxins acting on several nervous system targets but little is known about their antimicrobial and antiparasitic properties. Giardiasis, a common disease in developing countries, is produced by the protozoan Giardia duodenalis which induces severe gastroenteritis when infecting humans. The aim of this study is to describe the structural and molecular effects of Linuche unguiculata jellyfish extract on G. duodenalis. In this study, the effects on the ultrastructure were observed by electron transmission microscopy 24 h after the exposure to the extract and to the commercial antiparasitic drugs metronidazole, nitazoxanide and albendazole. To assess the effect at the molecular level, the gene expression analysis of β-tubulin, β-giardin, cysteine protease and glutamate dehydrogenase of G. duodenalis exposed to Linuche’s extract was performed by the RT-PCR technique. Our results show that the exposure to venom caused severe changes on Giardia’s cellular organelles such as submembrane vesicles, nuclei and adhesive disk. Our experiments also showed that the extract significantly alters the expression of genes that encode for the structural protein β-tubulin and the enzyme glutamate dehydrogenase, which are related to cell viability. Keywords Jellyfish . Venom . Antiprotozoal . Ultrastructure . Gene expression
Abbreviations GDH Glutamate dehydrogenase CP Cysteine protease RT-PCR Reverse Transcriptase-Polymerase Chain Reaction cDNA Complementary Deoxyribonucleic acid dNTP Deoxynucleotide triphosphate dATP Deoxyadenosine triphosphate dGTP Deoxyguanosine triphosphate dCTP Deoxycytidine triphosphate dTTP Deoxythymidine triphosphate * Judith Sánchez-Rodríguez [email protected] 1
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A. C., Subsede Noroeste, Parque de Investigación e Innovación Tecnológica (PIIT), Nuevo León Apodaca, Mexico
2
Unidad Académica de Sistemas Arrecifales Puerto Morelos, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Puerto Morelos, Quintana Roo, Mexico
3
Unidad Interinstitucional de Investigación Clínica y Epidemiológica, Facultad de Medicina, Universidad Autónoma de Yucatán, Mérida, Yucatán, Mexico
RNA HCl mM
Ribonucleic acid Hydrochloric acid Millimolar
Introduction Cnidarians (Hydrozoa, Scyphozoa, Cubozoa, Staurozoa and Anthozoa), have developed complex venoms capable of exerting powerful and specific actions against their prey or potential predators (Grimmelikhuijzen et al. 1996; Ball et al. 2004; Wiebring e
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