Unleash the Association of Mitochondrial Uncoupling Protein (UCP2) Promoter Variant (G-866A; rs659366) with Obesity: Ste
- PDF / 4,293,524 Bytes
- 33 Pages / 439.37 x 666.142 pts Page_size
- 0 Downloads / 130 Views
Unleash the Association of Mitochondrial Uncoupling Protein (UCP2) Promoter Variant (G‑866A; rs659366) with Obesity: Stepping from a Case–Control Study to a Meta‑analysis Hamada A. Abd El Daim1 · Afaf M. Elsaid2 · Amany A. Mousa3 · Mervat M. El‑Eshmawy3 · Lashin S. Lashin4,5 · Eman A. Toraih6,7 · Rami M. Elshazli8 Received: 8 March 2020 / Accepted: 22 May 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Numerous eligible articles investigated the potential impact of the promoter region of UCP2 (rs659366) variant and the susceptibility for obesity with questionable outcomes. Our team designed this case–control combined with meta-analysis survey to illustrate the contribution of this variant with obesity. This case–control survey was formulated based on 110 obese Egyptian patients and 122 non-obese controls. Genomic DNA was amplified for ascertaining of UCP2 (G-866A; rs659366) variant exploiting the PCR–RFLP technique. A literature search was completed to investigate the involvement of this variant with obesity from various genetic databases. In this case–control study, the distribution of UCP2 (rs659366) variant showed a significant association with obesity among Egyptian subjects under allelic and dominant models (P value = 0.0006 and A; rs659366), a missense variant within coding 55 (Ala55Val C>T; rs660339), and a 45 bp insertion/deletion variant (Qian et al. 2013; Zhang et al. 2014). Numerous reports studied the association of the promoter variant of UCP2 (G-866A; rs659366) with obesity susceptibility among numerous ethnic subjects but with insignificant conclusions (Brondani et al. 2014; Kring et al. 2008; Liu et al. 2013; Qian et al. 2013; Schauble et al. 2003). It is identified that G allele of the rs659366 variant within the UCP2 gene is associated with increased risk of obesity susceptibility, while the A allele is correlated with a reduced risk of obesity (Brondani et al. 2014; Esterbauer et al. 2001; Liu et al. 2013). Thus, our team designed this case-controlled study as an attempt to recognize the association of the promoter variant within the UCP2 (G-866A, rs659366) gene with obesity susceptibility among Egyptian subjects followed by a meta-analysis study to illustrate an accurate assessment of this association.
13
Biochemical Genetics
Subjects and Methods Case–Control Study Study Population Upon attaining an approval from the ethical and scientific committees of Horus and Mansoura Universities, Egypt, our team designed this current work in accordance with the declaration of Helsinki policies. Additionally, all participants were asked to assign a written consent before their admission in this survey. This casecontrolled survey was established based on 110 obese patients [Female/Male: 89 (80.9%)/21 (19.1%)], as well as another set of unrelated healthy volunteers as non-obese controls from the corresponding province within Egypt [Female/Male: 93 (76.2%)/29 (23.8%)]. All obese participants were recruited from the Outpatient’s Clinic of Specialized Medical Hospital, M
Data Loading...
