Up-regulation of GhPAP1A results in moderate anthocyanin accumulation and pigmentation in sub-red cotton
- PDF / 2,015,903 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 53 Downloads / 197 Views
ORIGINAL ARTICLE
Up‑regulation of GhPAP1A results in moderate anthocyanin accumulation and pigmentation in sub‑red cotton Aimin Liang1 · Jia Zhao1 · Xin Li1 · Fulin Yan1 · Zhong Chen1 · Xusheng Chen2 · Yi Wang1 · Yaohua Li1 · Chuannan Wang1 · Yuehua Xiao1 Received: 12 December 2019 / Accepted: 3 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Anthocyanins are a group of important secondary metabolites, functioning as colorant in plant organs as well as protective agents against several stresses. Sub-red plant (Rs) cottons, accumulating moderate level of anthocyanins in shoots, had increased photosynthesis efficiency compared to green- (GL) and red-plant (R1) cottons. The present work aimed to clarify the molecular base of anthocyanin regulation in Rs cotton. It was found that GhPAP1A was significantly up-regulated in Rs plants compared to GL cottons, but its expression level is lower than that of GhPAP1D in R1 plants. Virus induced gene silencing of GhPAP1s inhibited the red pigmentation in Rs plants. Comparative cloning revealed a 50-bp tandem repeat in the promoter of GhPAP1A in Rs cotton, which showed stronger activity to drive the expression of downstream genes in petals. Considered that the coding sequence of GhPAP1As from Rs and GL cottons had similar functions to promote anthocyanin biosynthesis in transgenic tobaccos, we attributed moderate anthocyanin accumulation in Rs cotton to increased transcription of GhPAP1A, resulted from varied promoter structure. Our works suggested GhPAP1s as useful tool to manipulate anthocyanin level and several breeding targets, including herbivore- and pathogen- resistance, high photosynthesis efficiency and colored fibers. Keywords Anthocyanin · Cotton · Sub-red plant · Rs gene · R2R3-MYB · Transcription factor · Promoter structure
Introduction Anthocyanins are major plant pigments coloring flowers, fruits, leaves, and other tissues. They may attract pollinators and seed dispersers, and protect plants from several stresses such as UV irradiation, low temperature, pathogen invasion and herbivore feeding (Long et al. 2019; Xu and Rothstein 2018; Liang and He 2018; Santos-Buelga et al. 2014; Nakabayashi et al. 2014). Anthocyanins were believed to be beneficial to human health (Kalt 2019; Butelli et al. 2008), and play an important role in enhancing the appearance, nutrient Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00438-020-01708-3) contains supplementary material, which is available to authorized users. * Yuehua Xiao [email protected] 1
Biotechnology Research Center, Southwest University, Chongqing, China
Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
2
quality, and thus commercial values of many agricultural products (Zhu et al. 2017). Meanwhile, anthocyanin and/ or its intermediates may interfere with normal plant growth and development (Shi and Xie 2014; Falcone Ferreyra et al. 2012; Kuhn et al. 2011; Tan et al. 2013). T
Data Loading...
