VrLELP controls flowering time under short-day conditions in Arabidopsis
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REGULAR PAPER – PHYSIOLOGY/BIOCHEMISTRY/MOLECULAR AND CELLULAR BIOLOGY
VrLELP controls flowering time under short‑day conditions in Arabidopsis Renxing Shi1 · Wenying Xu1 · Tong Liu1 · Chunmei Cai1 · Shuai Li1 Received: 8 June 2020 / Accepted: 15 October 2020 © The Botanical Society of Japan 2020
Abstract Flowering time has a critically important effect on the reproduction of plants, and many components involved in floweringtime regulation have been identified in multiple plant species. However, studies of the flowering-time genes in mungbean (Vigna radiata) have been limited. Here, we characterized a novel mungbean gene, VrLELP, involved in flowering-time regulation in transgenic Arabidopsis. Subcellular localization analysis revealed that VrLELP was localized in the membrane, cytoplasm and nucleus and the nucleus and membrane contained higher signal than cytoplasm, similar to the empty vector control. The expression of VrLELP was higher in leaves and pods and lower in nodule roots relative to other tissues. The expression of VrLELP varied during flower development. The expression of VrLELP also varied during the day, reaching a peak after 12 h of illumination under long-day conditions. In contrast, under short-day conditions, the abundance of VrLELP transcripts changed little throughout the day. In addition, VrLELP delayed flowering time in transgenic Arabidopsis plants by suppressing the expression of the flowering-time genes CO and FT under short-day conditions. However, VrLELP did not affect flowering time under long-day conditions in Arabidopsis. Our study provides essential information for future studies of the molecular mechanisms of the flowering-time regulation system in mungbean. Keywords Flowering time · Mungbean · Photoperiod · Short-day · VrLELP
Introduction Flowering time is an important factor for ensuring the reproductive success of crops, given that yields of crops are maximized when they flower at appropriate times. The transition from the vegetative to the reproductive stage is precisely regulated by the environment and endogenous cues in plants (Johansson and Staiger 2015; Wickland and Hanzawa 2015; Xu and Chong 2018). Among environmental factors, photoperiod is particularly important (Andres and Coupland 2012; Renxing Shi and Wenying Xu equally contributed to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10265-020-01235-7) contains supplementary material, which is available to authorized users. * Shuai Li [email protected] 1
College of Life Sciences, Key Lab of Plant Biotechnology in Universities of Shandong Province, Qingdao Agricultural University, Qingdao 266109, Shandong, China
Srikanth and Schmid 2011). Crops sense photoperiod signals and keep track of daylength and the day-night cycles through an endogenous timekeeper, the circadian clock, which is one of the most important internal cues determining flowering time (Dodd et al. 2005; Green et al. 2002). The measurement of photoperiodic time with the circadian c
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