Zone-Specific Changes in Micromechanical, Biochemical, and Structural Properties in Articular Cartilage from a Rabbit Jo
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Zone-Specific Changes in Micromechanical, Biochemical, and Structural Properties in Articular Cartilage from a Rabbit Joint Flexion Model Cheng Li1, Karen B. King2, and Lisa A. Pruitt1,3 1 UC Berkeley & UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley, 2121 Etcheverry Hall, Berkeley, CA, 94720 2 Department of Orthopaedics - Bioengineering, University of Colorado at Denver and Health Sciences Center, 12800 E. 19th Ave. RC1 N Room 2103, Mailstop 8343 PO Box 6511, Aurora, CO, 80045 3 Mechanical Engineering, University of California, Berkeley, 5134 Etcheverry Hall, Mailstop 1740, Berkeley, CA, 94720-1740 ABSTRACT Metacarpophalangeal (MCP) joint proximal bone-cartilage specimens from the fourth digit were collected from repetitively flexed and non-flexed (control) paws of four New Zealand White rabbits. The specimens were cryo-fractured to reveal a sagittal cut containing the cartilage zones of different collagen microstructure. Nanoindentation, Fourier Transform infrared microspectroscopic imaging (FTIRMI), and histology were performed on a region of interest (ROI) ~400 microns wide and through the thickness of the cartilage with two goals in mind: (1) to examine the effect of collagen network structure (random in the mid zone versus organized in the deep zone) on the biomechanical and biochemical properties of cartilage; and (2) to understand the changes in these properties due to physical forces. We found that zone microstructure significantly affected the measurement of the local relaxed modulus measured by nanoindentation. The deep zone had a higher modulus than the mid zone (Wilcoxon paired test, p
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