Quantification of Extracellular Proteases and Chitinases from Marine Bacteria
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Quantification of Extracellular Proteases and Chitinases from Marine Bacteria Yang Zou1,2 · Johan Robbens2 · Marc Heyndrickx2,3 · Jane Debode2 · Katleen Raes1 Received: 20 January 2020 / Accepted: 17 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A total of 92 marine bacteria belonging to Pseudomonas, Pseudoalteromonas, Psychrobacter, and Shewanella were first screened for their proteolytic activity. In total, four Pseudomonas strains belonging to Ps. fluorescens, Ps. fragi, Ps. gessardii, and Ps. marginalis; 14 Pseudoalteromonas strains belonging to Psa. arctica, Psa. carrageenovora, Psa. elyakovii, Psa. issachenkonii, Psa. rubra, Psa. translucida, and Psa. tunicata; and two Shewanella strains belonging to S. baltica and S. putrefaciens were identified to have a weak to high proteolytic activity (from 478 to 4445 mU/mg trypsin equivalent) against skim milk casein as protein source. Further chitinolytic activity screening based on these 20 proteolytic strains using colloidal chitin yielded five positive strains which were tested against three different chitin substrates in order to determine the various types of chitinases. Among the strains that can produce both proteases and chitinases, Psa. rubra DSM 6 842T expressed not only the highest proteolytic activity (2558 mU/mg trypsin equivalent) but also the highest activity of exochitinases, specifically, β-N-acetylglucosaminidase (6.33 mU/107 cfu) as well. We anticipate that this strain can be innovatively applied to the valorization of marine crustaceans side streams.
Introduction In the seafood industry, approximately 75% of the total weight of marine crustaceans ends up as side streams [1]. In the EU, these side streams were most of the time considered as waste and sent to landfill sites [2]. This behavior is considered a potentially large environmental hazard due to its abundant quantity. To overcome this problem, European legislation (EU Council Directive 1999/31/EC) has already Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02216-8) contains supplementary material, which is available to authorized users. * Katleen Raes [email protected] 1
Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University Campus Kortrijk, Graaf Karel de Goedelaan 5, 8500 Kortrijk, Belgium
2
Flanders Research Institute for Agriculture, Fisheries and Food, Burgemeester Van Gansberghelaan 92 bus 1, 9820 Merelbeke, Belgium
3
Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University Campus Merelbeke, Salisburylaan 133, 9820 Merelbeke, Belgium
set specific targets for the amount of Biodegradable Municipal Waste sent to landfill, which needs to be reduced with 75% of its 1995 levels by 2020. To reach this ambitious target, looking for alternatives to minimize the waste and to produce valuable compounds from these side streams was a hot topic in the past 20 years. Meanwhile,
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