Quantitative analysis of allelic differences in the grain proteome between the Wx g2 and Wx g3 alleles in rice ( Oryza s
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Quantitative analysis of allelic differences in the grain proteome between the Wxg2 and Wxg3 alleles in rice (Oryza sativa L.) Bin Teng
. Ying Zhang . Chen Zhang
Received: 10 June 2020 / Accepted: 19 August 2020 Ó Springer Nature B.V. 2020
Abstract The Wx gene is the major gene controlling amylose synthesis in rice endosperm. In a previous study, we were able to separate the Wxa allele into two functional Wx alleles, Wxg2 and Wxg3, according to their amylose phenotypes and a T/C nucleotide polymorphism in exon 10. Subsequent studies revealed that this Wx allelic variation also plays a vital role in regulating other important starch properties. However, the underlying molecular mechanisms related to the rice grain proteome have remained unclear. To elucidate this, we used two rice single-segment substitution lines harboring different Wx alleles, Wxg2 and Wxg3, in comparative proteomic studies. On the basis of iTRAQ quantitative proteomics, we identified a total of 185 proteins as differentially accumulated between the Wxg2 and Wxg3
lines. Gene Ontology functional analysis revealed that a high proportion of differentially expressed proteins (DEPs) are involved in metabolic and cellular biological processes. Further functional and pathway enrichment analyses indicated that relevant functions of the DEPs on protein degradation and binding may play important roles in distinguishing the Wxg2 and Wxg3 alleles. The present results may provide comprehensive insights into the molecular regulatory mechanisms of starch biosynthesis as influenced by the Wxg2 and Wxg3 alleles in rice.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10681-020-02685-9) contains supplementary material, which is available to authorized users.
Introduction
B. Teng (&) Y. Zhang Anhui Provincial Key Lab of Rice Genetics and Breeding, Institute of Rice Research, Anhui Academy of Agricultural Sciences, Hefei 230031, People’s Republic of China e-mail: [email protected] C. Zhang (&) College of Life Science, Anhui Agricultural University, Hefei 230061, People’s Republic of China e-mail: [email protected]
Keywords Rice Wx gene Allelic variation Quantitative proteomic Single-segment substitution line
Rice (Oryza sativa L.) is one of the most important food crops worldwide and is the staple food for about half of the global population (International Rice Genome Sequencing Project 2005). Starch is the major form of carbohydrate storage in the rice grain, and its physicochemical properties determine various aspects of rice quality. Starch is composed of amylose and amylopectin, two polymers of glucose, and the apparent amylose content (AAC) is recognized as one
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of the most important determinants of the cooking, processing, and eating qualities of rice (Juliano 1985). In rice endosperm, amylose synthesis is catalyzed by the granule-bound starch synthase (GBSS) enzyme that is encoded by the Waxy (Wx) gene (Wang et al. 1990). Sano
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