Reduction of cadmium accumulation in the grains of male sterile rice Chuang-5S carrying Pi48 or Pi49 through marker-assi
- PDF / 1,373,229 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 66 Downloads / 141 Views
ORIGINAL ARTICLE
Reduction of cadmium accumulation in the grains of male sterile rice Chuang‑5S carrying Pi48 or Pi49 through marker‑assisted selection Qiuhong Chen1 · Wei Tang1 · Gai Zeng1 · Haowen Sheng1 · Weijie Shi1 · Yinghui Xiao1,2 Received: 12 June 2018 / Accepted: 29 October 2020 / Published online: 18 November 2020 © King Abdulaziz City for Science and Technology 2020
Abstract To reduce cadmium (Cd) accumulation in the grains of rice Chuang-5S (C5S), a gene OsHMA3 and a QTL qlGCd3 related to low-Cd accumulation were separately introgressed into the recipient parent C5S (male sterile line) by molecular markerassisted breeding. The recurrent parent C5S was then replaced by NIL (near-isogenic line)-C5S with the blast resistance gene Pi48 or Pi49 to construct the BC2F1 generation. Finally, two groups of improved materials of C5S, which pyramided the gene/QTL associated with low-Cd accumulation and blast resistance gene, were developed. The Cd accumulation, agronomic traits, genetic background and blast resistance of these improved C5S materials were evaluated. The results showed that the average Cd content of improved C5S material carrying OsHMA3 and qlGCd3 was, respectively, reduced by 52.8% and 50.8% compared with that of C5S, indicating that the gene related to low-Cd accumulation was, successfully, transferred to C5S with stable expression. The main agronomic traits of the improved materials were consistent with those of C5S. Besides, the improved C5S lines showed stronger blast resistance than C5S and more than 88% similarity to the genetic background of C5S. These two groups of improved materials may be further utilized for the breeding of advanced male sterile lines or superior hybrid rice. Keywords Rice · Chuang-5S · Marker-assisted selection · Cadmium · Rice blast · Thermo-sensitive genic male sterile
Introduction Long-term consumption of rice with excess cadmium (Cd) can cause serious damage to human health (Ueno et al. 2010; Tang et al. 2017; Wang et al. 2019). Hence, various measures have been taken to alleviate Cd pollution in rice grains, such as the remediation of Cd-contaminated soil and improvement of cultivation techniques. However, the applicability of these methods is rather low due to their low efficiency or operability and high costs (Liu et al. 2016; Feng et al. 2019). Planting of rice varieties with low-Cd accumulation has been widely recognized as an effective and economic way Qiuhong Chen and Wei Tang have contributed equally to this work. * Yinghui Xiao [email protected] 1
Southern Regional Collaborative Innovation Center for Grain and Oil Crops, Hunan Agricultural University, Changsha 410128, China
Beidahuang Kenfeng Seed Co., Ltd., Harbin 150090, China
2
to reduce the Cd content in rice grains (Wang et al. 2016; Li et al. 2017; Chen et al. 2018; Luo et al. 2018). However, very few low-Cd accumulation rice varieties are available so far, which largely impedes the implementation of this method due to the following reasons (Zhao et al. 2018). First, the threat of exc
Data Loading...
