Serratia sp., an endophyte of Mimosa pudica nodules with nematicidal, antifungal activity and growth-promoting character
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ORIGINAL PAPER
Serratia sp., an endophyte of Mimosa pudica nodules with nematicidal, antifungal activity and growth‑promoting characteristics Erick Williams Méndez‑Santiago1,2 · Olga Gómez‑Rodríguez3 · Ricardo Sánchez‑Cruz1 · Jorge Luis Folch‑Mallol1 · Victor M. Hernández‑Velázquez1 · Edgar Villar‑Luna4 · Liliana Aguilar‑Marcelino2 · Arnoldo Wong‑Villarreal5 Received: 30 January 2020 / Revised: 3 August 2020 / Accepted: 15 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract In the present study, the nematicidal activity of an isolated strain of Mimosa pudica nodules was evaluated against the Nacobbus aberrans (J2) phytonymatodes with a mortality of 88.8%, while against the gastrointestinal nematode Haemonchus contortus (L3) and free-living Panagrellus redivivus was 100%. The ability to inhibit the growth of phytopathogenic fungi Fusarium sp., and Alternaria solani, as well as the oomycete Phytophthora capsici, this antifungal activity may be related to the ability to produce cellulases, siderophores and chitinases by this bacterial strain. Another important finding was the detection of plant growth promoter characteristics, such as auxin production and phosphate solubilization. The strain identified by sequences of the 16S and rpoB genes as Serratia sp. is genetically related to Serratia marcescens and Serratia nematodiphila. The promoter activity of plant growth, antifungal and nematicide of the Serratia sp. strain makes it an alternative for the biocontrol of fungi and nematodes that affect both the livestock and agricultural sectors, likewise, candidate as a growth-promoting bacterium. Keywords Solubilization phosphate · Serratia sp. · Nacobbus aberrans · Haemonchus contortus · Biocontrol
Introduction
Communicated by Jorge Membrillo Hernandes. * Liliana Aguilar‑Marcelino [email protected] * Arnoldo Wong‑Villarreal [email protected] 1
Centro de investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, , Morelos, México
2
Laboratorio de Helmintología, Centro Nacional de Investigación Disciplinaria en Salud Animal E Inocuidad, INIFAP, Carretera Federal Cuernavaca‑Cuautla No. 8534 Col. Progreso, C. P. 62550 Jiutepec, Morelos, México
3
Colegio de Postgraduados, Carretera México-Texcoco, Km. 36.5, Moncecillo, Texcoco, México
4
CONACYT-Instituto Politécnico Nacional, CIIDIR-IPN. Unidad Michoacán, Justo Sierra 28, 59510 Jiquilpan, Michoacán, México
5
División Agroalimentaria, Universidad Tecnológica de La Selva, Ocosingo, Chiapas, México
The fungal cell wall is basically constituted by chitin (Bartnicki-Garcia 1968), and in nematodes they have identified genes that code for chitin synthesis with differences between free-living and phytoparasites (Veronico et al. 2001), with highest content in the egg stage (Bird and Bird 1991). Chitin as a constituent of juveniles in phytopathogens has not been found (Veronico et al. 2001); however, in vitro tests Millew and Sands (1977) when applying chitinases achieved 100% control
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