Paraburkholderia guartelaensis sp. nov., a nitrogen-fixing species isolated from nodules of Mimosa gymnas in an ecotone
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ORIGINAL PAPER
Paraburkholderia guartelaensis sp. nov., a nitrogen‑fixing species isolated from nodules of Mimosa gymnas in an ecotone considered as a hotspot of biodiversity in Brazil Fabiane Paulitsch1,2,3 · Rebeca Fuzinatto Dall’Agnol3 · Jakeline Renata Marçon Delamuta4 · Renan Augusto Ribeiro4 · Jesiane Stefania da Silva Batista5 · Mariangela Hungria1,2 Received: 5 April 2019 / Revised: 14 July 2019 / Accepted: 10 August 2019 © Springer-Verlag GmbH Germany, part of Springer Nature 2019
Abstract A polyphasic approach was used to infer the phylogenetic position of six nitrogen-fixing symbiotic bacteria isolated from Mimosa gymnas nodules grown in an ecotone between the Brazilian biomes of Atlantic Forest and Cerrado, considered as a hotspot of biodiversity. The 16S rRNA gene phylogeny indicated the highest similarity with Paraburkholderia oxyphila (98.7–98.9%), but similar values were found with other Paraburkholderia species. The multilocus sequence analysis (MLSA) of five (recA, gyrB, trpB, gltB, and atpD) housekeeping genes indicated that the CNPSo strains represent a novel lineage, sharing less than 95.7% of nucleotide identity (NI) with other Paraburkholderia species, being more closely related to P. nodosa. Genome parameters were analyzed for strain CNPSo 3008T, and DNA–DNA hybridization revealed a maximum of 55.9% of DNA–DNA relatedness with P. nodosa, while average nucleotide identity with the two closest species was of 93.84% with P. nodosa and of 87.93% with P. mimosarum, both parameters confirming that the strain represents a new species. In the analysis of the nodulation nodC gene, all CNPSo strains showed the highest similarity with P. nodosa, and nodulation tests indicated host specificity with Mimosa. Other phylogenetic, physiological, and chemotaxonomic properties were evaluated. All data obtained support the description of the novel species Paraburkholderia guartelaensis sp. nov., with CNPSo 3008T (= U13000T = G29.01T) indicated as the type strain. Keywords Biological nitrogen fixation · Nodulation · MLSA · ANI · dDDH
Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-019-01714-z) contains supplementary material, which is available to authorized users. * Mariangela Hungria [email protected] Fabiane Paulitsch [email protected]
Abbreviations NI Nucleotide identity ANI Average nucleotide identity DDH DNA–DNA hybridization dDDH Digital DNA–DNA hybridization MLSA Multilocus sequence analysis
1
Embrapa Soja, C.P. 231, Londrina, Paraná 86001‑970, Brazil
2
Departamento de Microbiologia, Universidade Estadual de Londrina, C.P. 10011, Londrina, Paraná 86057‑970, Brazil
3
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, SBN, Quadra 2, Bloco L, Lote 06, Edifício Capes, Brasília, Distrito Federal 70040‑020, Brazil
4
Conselho Nacional de Desenvolvimento Científico E Tecnológico, SHIS QI 1 Conjunto B, Blocos A, B, C e D, Lago Sul, Brasília, Distrito Federal 7
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