Impedimetric aptasensor for Pseudomonas aeruginosa by using a glassy carbon electrode modified with silver nanoparticles
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ORIGINAL PAPER
Impedimetric aptasensor for Pseudomonas aeruginosa by using a glassy carbon electrode modified with silver nanoparticles Mahmoud Roushani 1 & Masoumeh Sarabaegi 1 & Fazal Pourahmad 2 Received: 12 May 2019 / Accepted: 19 September 2019 # Springer-Verlag GmbH Austria, part of Springer Nature 2019
Abstract An aptasensor is described for the ultrasensitive detection of Pseudomonas aeruginosa (P. aeruginosa). A glassy carbon electrode (GCE) was first modified by electrodeposition of silver nanoparticles (AgNPs). Immobilization of NH2-aptamer was covalently attached to the AgNP/GCE surface. The morphology, distribution and size of the sensor were characterized by field emission scanning electron microscopy. Cyclic voltammetry and electrical impedance spectroscopy were used to study conductivity of the aptasensor and the electrochemical properties. Detection of P. aeruginosa was carried out by evaluation of the charge transfer resistance after and before the adding of P.aeruginosa and by using the hexacyanoferrate redox system as an electrochemical probe. The impedance increases on going from 102 to 107 CFU·mL−1 concentrations of P. aeruginosa, and the detection limit is 33 CFU·mL−1 (for S/N = 3). The assay was successfully applied for the determination of P. aeruginosa in spiker serum samples. Keywords Aptamer . AgNPs . P. aeruginosa aptasensor . Polymerase chain reaction . Escherichia coli . Shigella flexneri . Salmonella enteritidis . Acinetobacter baumannii
Introduction P. aeruginosa can be present in foodstuff, water and feeds [1]. It has important effects on nosocomial infections in immuno-compromised patients with cancer, cystic fibrosis and lung disease [2, 3]. Several methods have attempted to find appropriate methods for P. aeruginosa detection. These analyses include polymerase chain reaction (PCR)-based methods [4], microbiological culture assay [5], immunology-based methods [6], Enzyme-linked immunosorbent assays
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-019-3858-y) contains supplementary material, which is available to authorized users. * Mahmoud Roushani [email protected]; [email protected] 1
Department of Chemistry, Ilam University, PO. Box 69315-516, Ilam, Iran
2
Department of Veterinary, Faculty of microbiology, University of Ilam, PO. Box 69315-516, Ilam, Iran
(ELISAs) [7], MALDI-TOF MS assays [8] and electrochemical biosensor [9]. Although, these methods are sensitive, but at the same time they are high cost, specialized facilities, complex working method and timeconsuming. Consequently, it is essential to develop new, simple, rapid, low-cost, and specific methods for P. aeruginosa detecting. Therefore, this study attempted to develop an aptasensor for achieving the precise detection of P.aeruginosa. Aptamer-based biosensors and assays have indicated greater advantages in pathogenic distinguishing. Aptamers that are short single- stranded DNA or RNA oligonucleotides have the ability of adopting special th
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