Cartilage Tissue Engineering Methods and Protocols
This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dime
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Pauline M. Doran Editor
Cartilage Tissue Engineering Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Cartilage Tissue Engineering Methods and Protocols
Edited by
Pauline M. Doran Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Melbourne, VIC, Australia
Editor Pauline M. Doran Faculty of Science, Engineering and Technology Swinburne University of Technology Hawthorn, Melbourne, VIC, Australia
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2937-5 ISBN 978-1-4939-2938-2 (eBook) DOI 10.1007/978-1-4939-2938-2 Library of Congress Control Number: 2015952786 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Cartilage in articular joints is a relatively vulnerable tissue, being subject to common injuries and degenerative conditions such as arthritis. Motivated by the need to develop new treatment strategies, some of the earliest attempts at tissue engineering targeted cartilage as a feasible goal for in vitro synthesis. For more than 20 years, interdisciplinary teams of biologists, engineers, materials scientists, and clinicians have studied the culture and differentiation of cartilage cells and tissues. Many cornerstone technologies that distinguish tissue engineering from routine cell culture, such as three-dimensional culture systems and the use of scaffolds and bioreactors, were developed, tested, and widely adopted within the context of cartilage tissue engineering. So far, the goal of producing laboratory-grown functional ca
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