Structural and functional characterization of bacterial cellulose from Enterobacter hormaechei subsp. steigerwaltii stra
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ORIGINAL RESEARCH
Structural and functional characterization of bacterial cellulose from Enterobacter hormaechei subsp. steigerwaltii strain ZKE7 Zabin K. Bagewadi . Venessa Dsouza . Sikandar I. Mulla . Sanjay H. Deshpande . Uday M. Muddapur . Deepak A. Yaraguppi . Vaddi Damodara Reddy . Jayateerth S. Bhavikatti . Sunil S. More
Received: 17 March 2020 / Accepted: 21 August 2020 Ó Springer Nature B.V. 2020
Abstract The present investigation reports, the bacterial cellulose (BC) a high-purity polymer produced from isolated strain ZKE7. BC production was optimized by Plackett–Burman and central composite designs yielding 18.5 g/l BC with a 4.5-fold enhancement. BC composites functionalized with antibiotics, BC-gelatin hydrogel, BC-Chitosan hydrogel and BC silver nanoparticle composites were developed and evaluated for water retention capability, moisture content and protein adsorption. The drug release
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10570-020-03412-2) contains supplementary material, which is available to authorized users. Z. K. Bagewadi (&) V. Dsouza S. H. Deshpande U. M. Muddapur D. A. Yaraguppi J. S. Bhavikatti Department of Biotechnology, KLE Technological University, Vidyanagar, Hubballi, Karnataka 580031, India e-mail: [email protected] S. I. Mulla V. D. Reddy Department of Biochemistry, School of Applied Sciences, REVA University, Bangalore 560064, India S. H. Deshpande ICMR-National Institute of Traditional Medicine, Belagavi, Karnataka 590010, India S. S. More School of Basic and Applied Sciences, Dayananda Sagar University, Bangalore 560078, India
behavior of the composites was consistent for controlled drug delivery. Composites were assessed for functional characteristic such as antimicrobial properties. BC composites functionalized with Neotericine exhibited antifungal activity against Candida albicans. Other composites showed pronounced antibacterial properties against Escherichia coli, Bacillus subtilis and Micrococcus luteus. Structural and thermal characterization of BC composites was carried out by FTIR, SEM with energy dispersive X-ray analysis, TGA and differential scanning calorimetry analysis. The results reveal high BC production with excellent properties that can be employed in biomedical field. Keywords Bacterial cellulose Enterobacter hormaechei Response surface methodology BC composites Antimicrobial Characterization
Introduction Cellulose is considered as an important biopolymer widely distributed and synthesized from different sources. Cellulose is a homopolymer of 1,4-linked bglucose residues. The plant cellulose is often accompanied by hemicellulose, pectin and lignin whereas, the bacterial cellulose (BC) is secreted in its pure form. Recovery of relatively pure cellulose from plants is challenging, but is less difficult for BC. Different bacteria produce cellulose in the form of water-
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Cellulose
insoluble pellicle with a 3D network of nanofiber
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