Study of Immunotropic Properties of Water-Soluble Polysaccharides Isolated from Conium maculatum Grass
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otropic Properties of Water-Soluble Polysaccharides Isolated from Conium maculatum Grass
A. A. Ligacheva1, E. Yu. Sherstoboev1, M. G. Danilets1, E. S. Trofimova1, S. V. Krivoshchekov2, A. M. Gur’ev2, T. V. Bulgakov3, N. V. Kudashkina3, A. G. Miroshnichenko2, and M. V. Belousov2 Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 8, pp. 168-172, August, 2020 Original article submitted April 8, 2020 Addition of water-soluble polysaccharides isolated from Conium maculatum L. to the mouse peritoneal macrophage culture induces classical activation of antigen-presenting cells due to an increase in NO synthase activity and a decrease in arginase expression. Key Words: plant polysaccharides; NO synthase; arginase
Polysaccharides of the bacterial wall can bind to pattern-recognizing structures of macrophages [7]. It has been shown that polysaccharides of microorganisms, fungi, and higher plants exhibit immunomodulating properties due to their ability to change the functional state of antigen-presenting cells (macrophages and dendritic cells) [3,4,6]. The immunomodulatory effect of plant polysaccharides on macrophages mainly consists in stimulation of ROS generation, cytokine secretion, cell proliferation, and phagocytic activity [11,14]. Nitric oxide (NO) is a neurotransmitter involved in numerous physiological and pathological processes of various systems, including immune responses and inflammation [15]. In the immune system, stimulation and activation of macrophages is accompanied by massive release of NO that can kill microorganisms, parasites, and tumor cells; it can also cause inflammatory reactions and protect the body from adverse external factors [12,13]. Our aim was to study the effect of water-soluble polysaccharides isolated from Conium maculatum L. 1 E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Aca demy of Sciences, Tomsk, Russia; 2Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia; 3Bashkir State Medical University, Ministry of Health of the Russian Fe deration, Ufa, Russia. Address for correspondence: sherstoboev_eu@ pharmso.ru. E. Yu. Sherstoboev
grass of the Apiaceae family on NO production by peritoneal macrophages (MP).
MATERIALS AND METHODS Peritoneal MP and lymphoid cells were isolated from female C57BL/6 mice (n=40) aged 6-8 weeks weighing 18-22 g (certified 1st category mice; Department of Experimental Biological Models, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine). All manipulations were carried out in accordance with the Directive 2010/63/EU of the European Parliament and Council (On the Protection of Animals used for Scientific Purposes; September 22, 2010) as well as GOST 33216-2014 Guidelines for the Maintenance and Care of Laboratory Animals. We used water-soluble polysaccharides (WSPS) isolated from Conium maculatum L. grass at the Department of Pharmaceutical Analysis of the Siberian State Medical University
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