Sulfate-reducing bacteria inhabiting natural corrosion deposits from marine steel structures
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ENVIRONMENTAL BIOTECHNOLOGY
Sulfate-reducing bacteria inhabiting natural corrosion deposits from marine steel structures Sandrine Païssé & Jean-François Ghiglione & Florence Marty & Ben Abbas & Hervé Gueuné & José Maria Sanchez Amaya & Gerard Muyzer & Laurent Quillet
Received: 4 July 2012 / Revised: 10 September 2012 / Accepted: 11 September 2012 / Published online: 20 October 2012 # Springer-Verlag Berlin Heidelberg 2012
Abstract In the present study, investigations were conducted on natural corrosion deposits to better understand the role of sulfate-reducing bacteria (SRB) in the accelerated corrosion process of carbon steel sheet piles in port environments. We describe the abundance and diversity of total and metabolically active SRB within five natural corrosion deposits located within tidal or low water zone and showing either normal or accelerated corrosion. By using molecular techniques, such as quantitative real-time polymerase chain reaction, denaturing gel gradient electrophoresis, and sequence cloning based on 16S rRNA, dsrB genes, and their transcripts, we demonstrated a clear distinction between SRB population structure inhabiting normal or
S. Païssé : L. Quillet LU Microbiologie Environnementale et Biologie Evolutive (MEBE), Université de Rouen, UFR des Sciences, IRESE B, Place Emile Blondel, Mont-Saint-Aignan 76821, France J.-F. Ghiglione CNRS, UMR7621, Laboratoire d’Océanographie Microbienne LOMIC, Banyuls-sur-Mer 66651, France J.-F. Ghiglione UPMC Univ Paris 06, UMR 7621, Laboratoire ARAGO, Banyuls-sur-Mer 66651, France
accelerated low-water corrosion deposits. Although SRB were present in both normal and accelerated low-water corrosion deposits, they dominated and were exclusively active in the inner and intermediate layers of accelerated corrosion deposits. We also highlighted that some of these SRB populations are specific to the accelerated low-water corrosion deposit environment in which they probably play a dominant role in the sulfured corrosion product enrichment. Keywords Accelerated low-water corrosion . Dissimilatory sulfite reductase . Microbiologically influenced corrosion . qPCR
J. M. S. Amaya Titania, Ensayos y Proyectos SL. Parque Tecnologico Tecnobahia, Edificio RETSE, Nave 4, Carretera Sanlucar Km.7, El Puerto de Santa Maria, Cadiz 11500, Spain
G. Muyzer Department of Aquatic Microbiology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam 1090, The Netherlands
F. Marty : B. Abbas : G. Muyzer Department of Biotechnology, Delft University of Technology, Delft 2628, The Netherlands H. Gueuné CORRODYS, Centre de Corrosion Marine et Biologique, Hall de Technologies, 145 Chemin de la Crespinière, BP 48, Cherbourg-Octeville 50130, France
Present Address: S. Païssé (*) LISBP-INSA Toulouse, EAD-9, 135 Avenue de Rangueil, Toulouse CEDEX 04 31077, France e-mail: [email protected]
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Introduction Accelerated corrosion of carbon steel is a long-standing problem for port authorities since premature or unexpected damages to infrastructures ca
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