The effects of everyday-life exposure to polycyclic aromatic hydrocarbons on biological age indicators
- PDF / 1,070,383 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 64 Downloads / 247 Views
RESEARCH
Open Access
The effects of everyday-life exposure to polycyclic aromatic hydrocarbons on biological age indicators Sofia Pavanello1,2* , Manuela Campisi1, Giuseppe Mastrangelo1, Mirjam Hoxha3 and Valentina Bollati3,4
Abstract Background: Further knowledge on modifiable aging risk factors is required to mitigate the increasing burden of age-related diseases in a rapidly growing global demographic of elderly individuals. We explored the effect of everyday exposure to polycyclic aromatic hydrocarbons (PAHs), which are fundamental constituents of air pollution, on cellular biological aging. This was determined via the analysis of leukocyte telomere length (LTL), mitochondrial DNA copy number (LmtDNAcn), and by the formation of anti-benzo[a]pyrene diolepoxide (B[a]PDE–DNA) adducts. Methods: The study population consisted of 585 individuals living in North-East Italy. PAH exposure (diet, indoor activities, outdoor activities, traffic, and residential exposure) and smoking behavior were assessed by questionnaire and anti-B[a]PDE–DNA by high-performance-liquid-chromatography. LTL, LmtDNAcn and genetic polymorphisms [glutathione S-transferase M1 and T1 (GSTM1; GSTT1)] were measured by polymerase chain reaction. Structural equation modelling analysis evaluated these complex relationships. Results: Anti-B[a]PDE–DNA enhanced with PAH exposure (p = 0.005) and active smoking (p = 0.0001), whereas decreased with detoxifying GSTM1 (p = 0.021) and in females (p = 0.0001). Subsequently, LTL and LmtDNAcn reduced with anti-B[a]PDE–DNA (p = 0.028 and p = 0.018), particularly in males (p = 0.006 and p = 0.0001). Only LTL shortened with age (p = 0.001) while elongated with active smoking (p = 0.0001). Besides this, the most significant determinants of PAH exposure that raised anti-B[a]PDE–DNA were indoor and diet (p = 0.0001), the least was outdoor (p = 0.003). Conclusion: New findings stemming from our study suggest that certain preventable everyday life exposures to PAHs reduce LTL and LmtDNAcn. In particular, the clear association with indoor activities, diet, and gender opens new perspectives for tailored preventive measures in age-related diseases. Capsule: Everyday life exposure to polycyclic aromatic hydrocarbons reduces leukocyte telomere length and mitochondrial DNA copy number through anti-B[a]PDE-DNA adduct formation. Keywords: Polycyclic aromatic hydrocarbon, Biological aging, Telomere length, Mitochondrial DNA copy number, DNA adduct, Structural equation modelling
* Correspondence: [email protected] 1 Medicina del Lavoro, Dipartimento di Scienze Cardio- Toraco- Vascolari e Sanità Pubblica, Università di Padova, Padova, Italy 2 Azienda Ospedaliera di Padova, Unità di Medicina del Lavoro, Padova, Italy Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give a
Data Loading...
