The Effects of Lactobacillus plantarum -12 Crude Exopolysaccharides on the Cell Proliferation and Apoptosis of Human Col
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The Effects of Lactobacillus plantarum-12 Crude Exopolysaccharides on the Cell Proliferation and Apoptosis of Human Colon Cancer (HT-29) Cells Mengying Sun 1 & Wenwen Liu 1 & Yinglong Song 1 & Yanfeng Tuo 1
&
Guangqing Mu 1,2 & Fenglian Ma 1
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The exopolysaccharide (EPS) of some Lactobacillus strains has been reported to exert anti-cancer activities. In this study, the effects of crude EPSs produced by four Lactobacillus plantarum strains (Lactobacillus plantarum-12, L. plantarum-14, L. plantarum-32, and L. plantarum-37) on HT-29 cell proliferation and apoptosis were studied. The results showed that the inhibition rate of the crude EPS produced by L. plantarum-12 on HT-29 cell proliferation was significantly higher than that of the EPS produced by the other three strains. L. plantarum-12 crude EPS (50, 100, 250, 500 μg/ml) exerted inhibitory effects on the expression of proliferating cell nuclear antigen (PCNA) in HT-29 cells in a positive dose-dependent manner. The reactive oxygen species (ROS) level and apoptosis rate were also increased in HT-29 cells treated with different concentrations of L. plantarum12 crude EPS compared with control cells. Further studies found that the expression of the pro-apoptotic proteins Bax, Cyt C, caspase-3, caspase-8 and caspase-9 was upregulated and that the expression of the anti-apoptosis protein Bcl-2 was decreased in HT-29 cells treated with L. plantarum-12 crude EPS compared with control cells. The results suggested that the EPS produced by L. plantarum-12 could inhibit the proliferation of the human colon cancer cell line HT-29 through the mitochondrial pathway. Keywords Lactobacillus . HT-29 cells . Proliferation . Apoptosis . Extracellular polysaccharide
Introduction Colorectal cancer (CRC) is one of the most common cancers worldwide. However, the incidence and mortality rate of CRC have decreased because of effective preventive measures and powerful treatment. Approximately 1,096,000 new cases of colon cancer were diagnosed in 2018, and CRC is the most diagnosed cancer among men in 10 of 191 countries worldwide [1, 2]. The incidence of colorectal cancer has been increasing globally. At the same time, the number of CRC patients has also gradually increased over the past decade in China [3]. The disruption of intestinal flora caused by poor dietary and lifestyle habits (i.e., intake of high-fat foods and lack of exercise) induces chronic inflammation, which
* Yanfeng Tuo [email protected] 1
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
2
Dalian Probiotics Function Research Key Laboratory, Dalian Polytechnic University, Dalian 116034, People’s Republic of China
eventually leads to cancer [4, 5]. The colon colonizes approximately 70% of the microbes present in the body, so an imbalance in intestinal flora is closely associated with colon cancer [6]. P r o b i o t i c s , i n c l u d i n g so m e Lact obacillus an d Bifidobacterium strains, are beli
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