The GATA Gene Family in Chickpea: Structure Analysis and Transcriptional Responses to Abscisic Acid and Dehydration Trea
- PDF / 2,193,380 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 53 Downloads / 128 Views
The GATAGene Family in Chickpea: Structure Analysis and Transcriptional Responses to Abscisic Acid and Dehydration Treatments Revealed Potential Genes Involved in Drought Adaptation Liangjie Niu1,2 · Ha Duc Chu3 · Cuong Duy Tran1,3 · Kien Huu Nguyen3 · Hoi Xuan Pham3 · Dung Tien Le4 · Weiqiang Li1,5 · Wei Wang2 · Thao Duc Le3 · Lam‑Son Phan Tran1,6 Received: 3 December 2019 / Accepted: 15 May 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The GATA transcription factors are important transcriptional regulators of plant growth and responses to environmental stimuli. Here, a total of 25 CaGATA genes was identified in chickpea (Cicer arietinum), and their basic characteristics, including gene structure, duplication patterns, conserved domains and various physical and chemical parameters were subsequently determined. Of our interest, the enrichment of the hormone- and stress-responsive cis-regulatory elements in the promoters of CaGATA genes has been analyzed to predict the CaGATA members with potential hormone-mediated functions in stress tolerance. Furthermore, the tissue-specific expression patterns of the CaGATAgenes were assessed using the available transcriptome data. More importantly, transcript levels of the identified CaGATA genes were quantified in roots and leaves of chickpea seedlings exposed to ABA (abscisic acid) or dehydration treatment using real-time quantitative PCR. Expression levels of a total of 12 CaGATA genes were significantly altered in roots and/or leaves by both ABA and dehydration treatments, suggesting that these genes might play roles in regulation of chickpea response to water stress in an ABA-dependent manner. Out of these genes, only CaGATA04 was induced in both roots and leaves by ABA and dehydration treatments. Furthermore, CaGATA05 and 21 were the most highly induced in roots (8.55-fold) and leaves (4.90-fold), respectively, by dehydration. Findings of this study have provided important insights into the CaGATAfamily of chickpea, as well as useful information for selection of CaGATAgenes of interest for in-depth functional characterizations that might lead to development of chickpea cultivars with improved performance under water-deficit conditions. Keywords Abscisic acid · Chickpea · Dehydration · Expression analysis · GATA · Transcription factors
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00344-020-10201-5) contains supplementary material, which is available to authorized users. * Thao Duc Le [email protected]
4
Faculty of Biotechnology, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam
* Lam‑Son Phan Tran [email protected]; [email protected]
5
State Key Laboratory of Cotton Biology, Henan Joint International Laboratory for Crop Multi‑Omics Research, State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Jinming Road, Kaifeng 475004, China
6
Institute of Research and Development, Duy Tan
Data Loading...
