The gut bacterial flora associated with brown planthopper is affected by host rice varieties
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ORIGINAL PAPER
The gut bacterial flora associated with brown planthopper is affected by host rice varieties Zheng‑Liang Wang1 · Hai‑bo Pan1 · Wei Wu1 · Mu‑Yu Li1 · Xiao‑Ping Yu1 Received: 22 June 2020 / Revised: 28 July 2020 / Accepted: 4 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Gut microbiota plays vital roles in the development, evolution and environmental adaptation of the host insects. The brown planthopper (BPH) is one of the most destructive pests of rice, but little is known about its gut microbiota. In this study, we investigated the gut bacterial communities in two BPH populations feeding on susceptible and resistant rice varieties by high-throughput amplicon sequencing. Our results revealed that the gut bacterial communities in BPH were species diverse. A total of 29 phyla and 367 genera were captured, with Proteobacteria and Acinetobacter being the most prominent phylum and genus, respectively. Comparative analysis showed that significant differences in the profile of gut bacterial communities existed between the two BPH populations. The species richness detected in the population feeding on the resistant rice variety was significantly higher than that in the population rearing on the susceptible rice variety. Although the most dominant gut bacteria at all taxonomic levels showed no significant differences between the two BPH populations, the relative abundances of two subdominant phyla (Firmicutes and Bacteroidetes) and two subdominant classes (Bacteroidia and Clostridia) were significantly different. FAPROTAX analysis further indicated that host rice varieties might induce changes of the gut bacterial flora in BPH, as significant differences in five metabolism-related functional categories (fermentation, methylotrophy, xylanolysis, nitrate reduction and ureolysis) were detected between the two BPH populations. Our results are informative for studies which focused on the interactions between BPH and its symbiotic microbes and could also provide the basis of future BPH biological management. Keywords Gut microbiota · Brown planthopper · Rice varieties · Amplicon sequencing
Introduction The brown planthopper (BPH), the common name for Nilaparvata lugens Stål (Hemiptera: Delphacidae), is one of the most destructive insect pests in paddy fields throughout Communicated by Erko Stackebrandt. Zheng-Liang Wang and Hai-bo Pan these two authors contributed equally to this paper. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02013-8) contains supplementary material, which is available to authorized users. * Xiao‑Ping Yu [email protected] 1
Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang, People’s Republic of China
Asia. As a monophagous insect herbivore of rice, BPH not only directly sucks sap from rice phloem through its stylet mouthpart resulting in serious wilt of the whole plant,
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