The Importance of Stem Cell Senescence in Regenerative Medicine
Mesenchymal stem cells (MSCs) are an interesting tool in regenerative medicine and a unique cell-based therapy to treat aging-associated diseases. Successful MSC therapy needs a large-scale cell culture, and requires a prolonged in vitro cell culture that
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The Importance of Stem Cell Senescence in Regenerative Medicine Maliheh Khademi-Shirvan, Mahsa Ghorbaninejad, Samaneh Hosseini, and Mohamadreza Baghaban Eslaminejad
Abstract
Mesenchymal stem cells (MSCs) are an interesting tool in regenerative medicine and a unique cell-based therapy to treat agingassociated diseases. Successful MSC therapy needs a large-scale cell culture, and requires a prolonged in vitro cell culture that subsequently leads to cell senescence. Administration of senescent MSCs results in inefficient cell differentiation in the clinical setting. Therefore, it is of utmost importance to enhance our knowledge about the aging process and methods to detect cell senescence in
order to overcome this challenge. Numerous studies have addressed senescence in various aspects. Here, we review the characteristics of MSCs, how aging affects their features, mechanisms involved in aging of MSCs, and potential approaches to detect MSC senescence in vitro. Keywords
Aging · Mesenchymal stem cell · Regenerative medicine
Abbreviations Authors Maliheh Khademi-Shirvan and Mahsa Ghorbaninejad have equally contributed to this chapter M. Khademi-Shirvan Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran M. Ghorbaninejad Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran S. Hosseini (*) and M. Baghaban Eslaminejad (*) Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran e-mail: [email protected]; [email protected]
5-AzaC AD-MSCs array-CGH BMSCs CDKN2B CFU-F CNAs DNMT FlowFISH GVHD HATs HDACs HDMs
5-azacytidine adipose-derived MSCs array complete genomic hybridization bone marrow MSCs cyclin-dependent kinase inhibitor 2B colony-forming unit fibroblasts copy number alterations DNA methyltransferases flow fluorescence in situ hybridization graft-versus-host disease histone acetyltransferase histone deacetylases histone demethylases
M. Khademi-Shirvan et al.
hMSCS HMTs hTERC hTERT ISCT mBMSCs MSCs mTRF PARG1 PcG PD SADNAm SA-β-Gal siRNA SNVs
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human MSCs histone methyltransferases telomerase RNA template catalytic enzyme telomerase reverse transcriptase international society of cellular therapy mouse BMSCs mesenchymal stem cells mean telomere restriction fragment phosphate-associated RhoGAP protein-tyrosine polycomb-group population doubling senescence-associated DNA-methylation senescence-associated betagalactosidase small interfering RNA single nucleotide variations
Introduction
Regenerative medicine is the most recent, modern branch of biomedical sciences and mesenchymal stem cells (MSCs) constitute one of i
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