The secretions of oviduct epithelial cells increase the equine in vitro fertilization rate: are osteopontin, atrial natr
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The secretions of oviduct epithelial cells increase the equine in vitro fertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved? Sylvie Mugnier1,2,3,4, Morgane Kervella1,2,3,4, Cécile Douet1,2,3,4, Sylvie Canepa1,2,3,4, Géraldine Pascal1,2,3,4, Stefan Deleuze5, Guy Duchamp6, Philippe Monget1,2,3,4 and Ghylène Goudet*1,2,3,4 Address: 1INRA, UMR85, Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France, 2CNRS, UMR6175, F-37380 Nouzilly, France, 3Université François Rabelais de Tours, F-37041 Tours, France, 4Haras Nationaux, F-37380 Nouzilly, France, 5Faculté de Médecine Vétérinaire, Département des Sciences Cliniques - Clinique Equine, Université de Liège, B-4000 Liège, Belgium and 6INRA, UE1297 Unité Expérimentale de Physiologie Animale de l'Orfrasière, F-37380 Nouzilly, France Email: Sylvie Mugnier - [email protected]; Morgane Kervella - [email protected]; Cécile Douet - [email protected]; Sylvie Canepa - [email protected]; Géraldine Pascal - [email protected]; Stefan Deleuze - [email protected]; Guy Duchamp - [email protected]; Philippe Monget - [email protected]; Ghylène Goudet* - [email protected] * Corresponding author
Published: 19 November 2009 Reproductive Biology and Endocrinology 2009, 7:129
doi:10.1186/1477-7827-7-129
Received: 7 September 2009 Accepted: 19 November 2009
This article is available from: http://www.rbej.com/content/7/1/129 © 2009 Mugnier et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Oviduct epithelial cells (OEC) co-culture promotes in vitro fertilization (IVF) in human, bovine and porcine species, but no data are available from equine species. Yet, despite numerous attempts, equine IVF rates remain low. Our first aim was to verify a beneficial effect of the OEC on equine IVF. In mammals, oviductal proteins have been shown to interact with gametes and play a role in fertilization. Thus, our second aim was to identify the proteins involved in fertilization in the horse. Methods & results: In the first experiment, we co-incubated fresh equine spermatozoa treated with calcium ionophore and in vitro matured equine oocytes with or without porcine OEC. We showed that the presence of OEC increases the IVF rates. In the subsequent experiments, we coincubated equine gametes with OEC and we showed that the IVF rates were not significantly different between 1) gametes co-incubated with equine vs porcine OEC, 2) intact cumulus-oocyte complexes vs denuded oocytes, 3) OEC previously stimulated with human Chorionic Gonadotropin, Luteinizing Hormone and/or oestradiol vs non stimulated OEC, 4) in vivo vs in vitro matured oocytes. In order to identify the
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