Thiol-suppressed I 2 -etching of AuNRs: acetylcholinesterase-mediated colorimetric detection of organophosphorus pestici
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ORIGINAL PAPER
Thiol-suppressed I2-etching of AuNRs: acetylcholinesterase-mediated colorimetric detection of organophosphorus pesticides Zhihe Qing 1,2 & Yacheng Li 1 & Younan Li 1 & Guoyan Luo 1 & Jinlei Hu 1 & Zhen Zou 1 & Yanli Lei 1 & Juewen Liu 2 & Ronghua Yang 1,3 Received: 1 February 2020 / Accepted: 6 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract For the first time it is demonstrated that sulfhydryl compounds can suppress longitudinal etching of gold nanorods via consuming oxidizers, which provides a new signaling mechanism for colorimetric sensing. As a proof of concept, a colorimetric assay is developed for detecting organophosphorus pesticides, which are most widely used in modern agriculture to improve food production but with high toxicity to animals and the ecological environment. Triazophos was selected as a model organophosphorus pesticide. In the absence of triazophos, the active acetylcholinesterase can catalyze the conversion of acetylthiocholine iodide to thiocholine whose thiol group can suppress the I2-induced etching of gold nanorods. When triazophos is present, the activity of AchE is inhibited, and I2-induced etching of gold nanorods results in triazophos concentration-dependent color change from brown to blue, pink, and red. The aspect ratio of gold nanorods reduced with gradually blue-shifted longitudinal absorption. There was a linear detection range from 0 to 117 nM (R2 = 0.9908), the detection limit was 4.69 nM, and a good application potential was demonstrated by the assay of real water samples. This method will not only contribute to public monitoring of organophosphorus pesticides but also has verified a new signaling mechanism which will open up a new path to develop colorimetric detection methods. Keywords Gold nanorods . Iodine etching . Colorimetric assay . Organophosphorus pesticides . Triazophos
Introduction Yacheng Li and Younan Li contributed equally as co-first authors. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04486-2) contains supplementary material, which is available to authorized users. * Zhihe Qing [email protected] * Ronghua Yang [email protected] 1
Hunan Provincial Key Laboratory of Cytochemistry, Hunan Provincial Engineering Research Center for Food Processing of Aquatic Biotic Resources, School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410114, People’s Republic of China
2
Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L3G1, Canada
3
Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China
In modern agriculture, pesticides have been widely used to increase food production [1, 2]. However, pesticides often result in pollution in the environment and harm to human health [3, 4]. Organophosphorus pesticides (OPs) are most
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