Transcriptional reprogramming of major defense-signaling pathways during defense priming and sugarcane- Colletotrichum f
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ORIGINAL ARTICLE
Transcriptional reprogramming of major defense‑signaling pathways during defense priming and sugarcane‑Colletotrichum falcatum interaction N. M. R. Ashwin1 · Leonard Barnabas1,2 · Dharmaraj Amalamol1 · Kana Valiyaveettil Lakshana1 · Amalraj Ramesh Sundar1 · Palaniyandi Malathi1 · Rasappa Viswanathan1 Received: 16 June 2020 / Accepted: 22 October 2020 © Springer Nature B.V. 2020
Abstract Red rot caused by Colletotrichum falcatum poses a serious threat to sugarcane cultivation in many tropical and sub-tropical countries. Deciphering the molecular network of major defense-signaling pathways in sugarcane cultivars with varying red rot resistance is essential to elucidate the phenomenon of defense priming exerted by resistance inducers. Therefore, in this study, expression pattern of transcripts coding for major defense-signaling pathway regulatory genes was profiled during compatible and incompatible interactions and in response to defense priming using qRT-PCR. Candidate genes that were profiled are involved in or related to hypersensitive response and reactive oxygen species production (HR/ROS), salicylic acid (SA), and jasmonic acid/ethylene (JA/ET) pathways. For compatible and incompatible interactions, susceptible (CoC 671), field tolerant (Co 86032) and resistant (Co 93009) sugarcane cultivars were used, whereas for defense priming, benzothiadiazole (BTH) and the pathogen-associated molecular patterns (PAMPs) of C. falcatum viz., CfEPL1 (eliciting plant response-like) and CfPDIP1 (plant defense inducing protein) were used in CoC 671 cultivar. Results indicated that the master regulator of defense pathways, nonexpressor of pathogenesis-related genes 1 (NPR1) was highly upregulated in incompatible interactions (in both Co 86032 and Co 93009) than the compatible interaction along with SA pathway-associated genes. Similarly, in response to defense priming with BTH, CfEPL1 and CfPDIP1, only the SA pathway-associated genes showed considerable upregulation at 0 h post inoculation (hpi) and other intermittent time points. Overall, this study showed that SA-mediated defense pathway is the most predominant pathway reprogrammed during priming with BTH, CfEPL1 and CfPDIP1 and substantiated the earlier findings that these agents indeed induce systemic resistance against red rot of sugarcane. Keywords Sugarcane · Priming · Colletotrichum falcatum · Defense-related genes · CfEPL1 · CfPDIP1
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05944-z) contains supplementary material, which is available to authorized users.
Red rot caused by the fungus Colletotrichum falcatum is one of the serious problems for sugarcane production in many tropical countries. Owing to the economic significance of
* Amalraj Ramesh Sundar [email protected]; [email protected]
Palaniyandi Malathi [email protected]
N. M. R. Ashwin [email protected]
Rasappa Viswanathan [email protected]
Leonard Barnabas leonardbarn
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