Effects of Glucose Assimilation on Lutein and Chlorophyll Biosyntheses in the Green Alga Chlorella pyrenoidosa
Glucose assimilation caused a significant decrease of lutein and chlorophyll accumulation in Chlorella pyrenoidosa cultivated in low nitrogen medium, which are in line with the previously reported glucose-bleaching effect on the green alga Chlorella proto
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Effects of Glucose Assimilation on Lutein and Chlorophyll Biosyntheses in the Green Alga Chlorella pyrenoidosa Tao Li, Yi-han Liu, Fu-ping Lu and Yue Jiang
Abstract Glucose assimilation caused a significant decrease of lutein and chlorophyll accumulation in Chlorella pyrenoidosa cultivated in low nitrogen medium, which are in line with the previously reported glucose-bleaching effect on the green alga Chlorella protothecoides cultivated in nitrogen deficient medium. Our study showed that an excessive lipid production induced by nitrogen deficiency was expected to be responsible for the decrease of lutein and chlorophyll accumulation in C. pyrenoidosa under heterotrophic conditions. When C. pyrenoidosa was cultivated in high nitrogen medium, the lutein and chlorophyll biosyntheses acted very differently: lutein content still decreased markedly whereas chlorophyll content remained stable. In addition, when we inhibited chlorophyll biosynthesis with levulinic acid, the chlorophyll a (chl a) and chlorophyll b (chl b) contents decreased markedly, but the lutein biosynthesis was not influenced at all. It was concluded that lutein and chlorophyll biosyntheses were possibly not coordinated in C. pyrenoidosa under heterotrophic conditions. Keywords Lutein
Chlorophyll Chlorella pyrenoidosa Heterotrophic
T. Li Basic Science Department, Tianjin Agricultural University, Tianjin 300384, People’s Republic of China T. Li Y. Jiang (&) Department of Biology and Kwong Living Trust Food Safety and Analysis Lab, Hong Kong Baptist University, Kowloon Tong, Hong Kong, People’s Republic of China e-mail: [email protected] Y. Liu F. Lu The College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People’s Republic of China
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 250, DOI: 10.1007/978-3-642-37922-2_101, Ó Springer-Verlag Berlin Heidelberg 2014
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101.1 Introduction Chlorella pyrenoidosa is a nonmotile, unicellular freshwater green alga [1]. It can grow not only under photoautotrophic conditions but also in darkness by making use of glucose as carbon and energy sources. Lutein, chlorophyll a (chl a), chlorophyll b (chl b), and other photosynthetic pigments can also be produced by C. pyrenoidosa under darkness [2–5]. In plant and green alga, lutein, and other carotenoids bind the light harvesting complexes which are located in the thylakoids membrane of chloroplast. So the biosynthesis of carotenoid must be coordinated with the formation of other photosynthetic components [6]. Chlorophyll and lutein biosyntheses are coordinated and regulated through the action of phytochrome and blue/UV receptors in plant and green alga under photoautotrophic conditions [7]. Besides, the presence of chlorophyll is prerequisite for the biosynthesis of carotenoid in the plastid of Sinapis alba [8]. So it was reported that the accumulation of carotenoids was inhibited when the chlo
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