Transcriptome assembly and expression profiling of the molecular responses to cadmium toxicity in cerebral ganglia of wo

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Transcriptome assembly and expression profiling of the molecular responses to cadmium toxicity in cerebral ganglia of wolf spider Pardosa pseudoannulata (Araneae: Lycosidae) Huilin Yang1,2 Yuande Peng3 Yixue Shi2 Jianxiang Tian4 Juan Wang2 Xianjin Peng5 Chunliang Xie3 Xiang Xu5 Qisheng Song6 Zhi Wang2 Zhiyue Lv7 ●



















Accepted: 5 December 2017 © The Author(s) 2018. This article is an open access publication

Abstract Cadmium (Cd) is a heavy metal that can cause irreversible toxicity to animals, and is an environmental pollutant in farmlands. Spiders are considered to be an excellent model for investigating the impacts of heavy metals on the environment. To date, the changes at the molecular level in the cerebral ganglia of spiders are poorly understood. Cd exposure leads to strong damage in the nervous system, such as apoptosis and necrosis of nerve cells, therefore we conducted a transcriptomic analysis of Pardosa pseudoannulata cerebral ganglia under Cd stress to profile differential gene expression (DGE). We obtained a

Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10646-017-1885-1) contains supplementary material, which is available to authorized users. * Zhi Wang [email protected] * Zhiyue Lv [email protected] 1

College of Orient Science & Technology, Hunan Agriculture University, No. 1 Nongda Road, Changsha 410128 Hunan, China

2

College of Bioscience and Biotechnology, Hunan Agriculture University, No. 1 Nongda Road, Changsha 410128 Hunan, China

3

Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205 Hunan China

4

College of Continuing Education, Hunan Agriculture University, No. 1 Nongda Road, Changsha 410128 Hunan, China

5

College of Life Science, Hunan Normal University, Changsha 410006 Hunan, China

6

Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA

7

Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China

total of 123,328 assembled unigenes, and 1441 Cd stressassociated DEGs between the Cd-treated and control groups. Expression profile analysis demonstrated that many genes involved in calcium signaling, cGMP—PKG signaling, tyrosine metabolism, phototransduction–fly, melanogenesis and isoquinoline alkaloid biosynthesis were upregulated under Cd stress, whereas oxidative phosphorylation-related, nervous disease-associated, non-alcoholic fatty liver disease-associated, and ribosomal-associated genes were down-regulated. Here, we provide a comprehensive set of DEGs influenced by Cd stress, and heavy metal stress, and provide new information for elucidating the neurotoxic mechanisms of Cd stress in spiders. Keywords Cadmium Transcriptomic P. pseudoannulata Cerebral ganglion ●





Introduction Cadmium (Cd) is a non-essential heavy metal that is a nonbiodegradable pollutant across the world (Mehinto et al. 2014; Nemmiche et al. 2011). Cd is highly neurotoxic in animals, and damages induced by high level