Transcriptome exploration to provide a resource for the study of Auricularia heimuer
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ORIGINAL PAPER
Transcriptome exploration to provide a resource for the study of Auricularia heimuer Jian Zhang1 • Tingting Sun2 • Shixin Wang1 • Li Zou1
Received: 21 November 2018 / Accepted: 21 February 2019 Ó Northeast Forestry University 2019
Abstract Auricularia heimuer, an edible jelly fungus, is in considerable demand in Asia due to its high nutritive, economic and medicinal values. RNA-Seq was used to investigate and analyze the mycelium transcriptome of A. heimuer for gene discovery. A total of 26,857 unigenes with an N50 length of 1333 bp were assembled by de novo sequencing. In addition, unigenes were annotated by publicly available databases, including gene descriptions, gene ontology (GO), clusters of orthologous group (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways, and protein family (Pfam) terms. A. heimuer was also studied for its wood degradation ability. Thirty-eight putative FOLymes (fungal oxidative lignin enzymes) and 251 CAZymes (carbohydrate-active enzymes) were located from A. heimuer transcriptome. Our study provides a comprehensive sequence resource for A. heimuer at the transcriptional level, which will lay a strong Project funding: The work was supported by the Fundamental Research Funds for the Central Universities, (Project No. 2572017CF01) and the Harbin University Scientific Research Foundation project, (Project No. HUDF2018105). The online version is available at https://www.springerlink.com Corresponding editor: Tao Xu.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11676-019-00989-8) contains supplementary material, which is available to authorized users. & Li Zou [email protected] 1
College of Forestry, Northeast Forestry University, Harbin 150040, People’s Republic of China
2
Department of Food Engineering, Harbin University, Harbin 150086, People’s Republic of China
foundation for functional genomics studies and gene discovery of this promising fungus. Keywords Auricularia heimuer Transcriptome Carbohydrate active enzymes Lignin degradation Nextgeneration sequencing
Introduction Auricularia heimuer F.Wu, B.K.Cui & Y.C.Dai, an ear-like shaped macro-fungus, is one of the most widely cultivated edible mushrooms in China due to its nutritional and medicinal properties (Fan et al. 2014). It has potential antitumor, anti-inflammatory, anticoagulant and hypoglycemic activities (Misaki et al. 1981; Ukai et al. 1983; Yuan et al. 1998; Yoon et al. 2003). Furthermore, its extracts have been reported to possess antioxidant and nitric oxide synthase activation properties (Acharya et al. 2004). A.heimuer is a white rot fungus which absorbs its nutrients by degrading lignin, cellulose and hemicellulose. In recent years, with the increasing demand of A.heimuer both at home and abroad, large numbers of trees are utilized to cultivate this fungus. However, the cultivation of A.heimuer has not been reported at a molecular level due to insufficient genomic sequence resources. Over the past several years, ne
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