Molecular characteristics of a novel ssRNA virus isolated from Auricularia heimuer in China
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Molecular characteristics of a novel ssRNA virus isolated from Auricularia heimuer in China Xuefei Li1,2 · Jiatao Xie2 · Du Hai2 · Kunpeng Sui1 · Weiqi Yin1 · Frederick Leo Sossah1 · Daohong Jiang2 · Bing Song1 · Yu Li1 Received: 25 February 2020 / Accepted: 16 March 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A novel positive-sense single-stranded RNA virus was isolated from strain CCMJ1271 of the fungus Auricularia heimuer, and the complete genome sequence of the virus was determined. Database searching, sequence alignment, and phylogenetic analysis revealed that this fungal virus and some viruses of family Virgaviridae clustered into a single branch of a phylogenetic tree, and we thus tentatively named the virus "Auricularia heimuer mycovirgavirus 1" (AhMV1). The AhMV1 genome consists of 9,934 nucleotides and contains a short poly(A) tail and three open reading frames (ORFs). ORF1 encodes an RNA-dependent RNA polymerase (RdRp), ORF2 encodes a protein that is homologous to movement proteins of plant virgaviruses, and ORF3 encodes a coat protein (CP). AhMV1 is the first virus to be discovered in A. heimuer. Mycoviruses infect fungi, subsequently replicating and stably reproducing within their particular host. Fungal viruses have been found in edible and medicinal fungi, plant endophytic fungi, and phytopathogenic fungi [1]. Fungal viruses have received significant attention in recent years, and related research studies have been conducted [2–4]. In 1962, Hollings et al. identified three different viruses that infected experimentally inoculated fungi [2, 3, 5, 6]. Most of the reported fungal viruses have a double-stranded RNA (dsRNA) or positive-sense (+) singlestranded RNA (ssRNA) genome, but a few fungal viruses Handling Editor: Robert H. A. Coutts. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04615-5) contains supplementary material, which is available to authorized users. * Bing Song [email protected] * Yu Li [email protected] 1
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin, China
State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
2
have a negative-sense (−) ssRNA or single-stranded DNA (ssDNA) genome [7–14]. These studies form the basis for analyzing the pathogenic mechanisms and diversity of the viruses that infect edible fungi. According to the Report of the 10th International Committee on Taxonomy of Viruses (ICTV), viruses of the family Virgaviridae are divided into seven genera, namely, Furovirus, Goravirus, Hordeivirus, Pecluvirus, Pomovirus, Tobamovirus, and Tobravirus [15]. These viruses have a (+) ssRNA genome of 6.3–13 kb [15, 19], and their virions, which have no envelope, are about 20 nm in diameter and 300 nm in length [15, 19], altho
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