Ultrasonically Assisted Extraction of Rutin from Artemisia selengensis Turcz: Comparison with Conventional Extraction Te
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Ultrasonically Assisted Extraction of Rutin from Artemisia selengensis Turcz: Comparison with Conventional Extraction Techniques Lin Peng & Xiaopin Jia & Yuzhi Wang & Hongbin Zhu & Qingmei Chen
Received: 25 June 2009 / Accepted: 31 August 2009 / Published online: 17 September 2009 # Springer Science + Business Media, LLC 2009
Abstract Ultrasonically assisted extraction (UAE) followed by high performance liquid chromatography (HPLC) analysis method for the fast extraction and determination of rutin in Artemisia selengensis Turcz has been developed. Artemisia selengensis Turcz has been used as food and herbal medicine for thousands of years in China. Rutin is one of the main active ingredients of this plant. The extraction of rutin from Artemisia selengensis Turcz was investigated by UAE. Special emphasis has been given to optimize the extraction conditions which were those with 90:10 (v/v) methanol–ethanol as solvent, 30:1 liquid–solid ratio, and 40 min extraction time. In order to show the superiority of UAE, other extractions were investigated, including microwave-assisted extraction, reflux extraction, and marinated extraction. The results showed that UAE was most suitable for the extraction of rutin in Artemisia selengensis Turcz because of its high extraction efficiency. Reversed phase-HPLC with ultraviolet detection was employed for the analysis of rutin in Artemisia selengensis Turcz. Under the optimum conditions, the calibration curve for the analyte was linear in the range of 0.34–20.7 μg mL−1. The mean recovery of rutin was 100.77%, and its relative standard deviation was 0.37% (n=5). Three kinds of Artemisia selengensis Turcz from different habitats were investigated. The total content of rutin was 9.90, 6.23, 5.56 mg g−1, respectively.
L. Peng : X. Jia : Y. Wang (*) : H. Zhu : Q. Chen State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People’s Republic of China e-mail: [email protected]
Keywords Food Analysis . Artemisia selengensis Turcz . Rutin . Ultrasonically Assisted Extraction . HPLC
Introduction Artemisia selengensis Turcz (Chinese name: Li Hao; Zhong and Qing 2006) is a common, herbaceous succulent annual weed, which is distributed in low-lying wet district, marshes, and freshwater lake. It has been used as food and herbal medicine for thousands of years in China. Artemisia selengensis Turcz has been used traditionally to stop bleeding, diminish inflammation, relieve cough, and stimulate appetite (Zhang et al. 2004). Among plant metabolites of many Chinese herbal medicines, rutin (3’, 4’, 5, 7-tetrahydroxyflavone-3-β-Drutinoside) is one of the most bioactive flavonol glycoside plant metabolite. Rutin has received much attention because of its pharmaceutical use in phytotherapy. Its therapeutic action is mainly related to the heart and the kidney. It has also been reported as antimicrobial and antihypertensive (Yang and Zhang 2008). It has been reported that rutin is present in Artemisia selengensis Turcz(Zhang
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