Yersinia Pestis Protocols
This protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, pro
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Yersinia Pestis Protocols
SPRINGER PROTOCOLS HANDBOOKS
For further volumes: http://www.springer.com/series/8623
Yersinia Pestis Protocols Edited by
Ruifu Yang Beijing Institute of Microbiology and Epidemiology Beijing, China
Editor Ruifu Yang Beijing Institute of Microbiology and Epidemiology Beijing, China
ISSN 1949-2448 ISSN 1949-2456 (electronic) Springer Protocols Handbooks ISBN 978-981-10-7946-7 ISBN 978-981-10-7947-4 (eBook) https://doi.org/10.1007/978-981-10-7947-4 Library of Congress Control Number: 2018939546 © Springer Nature Singapore Pte Ltd. 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Springer imprint is published by the registered company Springer Nature Singapore Pte Ltd. The registered company address is: 152 Beach Road, #21-01/04 Gateway East, Singapore 189721, Singapore
Contents 1 Isolation and Identification of Yersinia pestis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Zhizhen Qi, Qingwen Zhang, Yong Jin, Youquan Xin, Haihong Zhao, Xiaoyan Yang, Xiaoqing Xu, and Xuefei Zhang 2 Isolation and Characterization of Yersinia pestis Bacteriophages. . . . . . . . . . . . . . . Xiangna Zhao 3 Extraction of Chromosomal DNA and Plasmid from Yersinia pestis . . . . . . . . . . . Yazhou Zhou 4 Extraction of Total RNA from Yersinia pestis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Haili Wang and Yanping Han 5 Genotyping of Yersinia pestis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Yanjun Li and Yujun Cui 6 Genome-Wide Variation Analysis of Yersinia pestis . . . . . . . . . . . . . . . . . . . . . . . . . . Chao Yang and Yujun Cui 7 Genome-Wide Detection of Expressed sRNAs in Yersinia pestis with RNA-seq . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Yanpi
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