SOS5 gene-abscisic acid crosstalk and their interaction with antioxidant system in Arabidopsis thaliana under salt stres
- PDF / 1,755,667 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 104 Downloads / 168 Views
RESEARCH ARTICLE
SOS5 gene-abscisic acid crosstalk and their interaction with antioxidant system in Arabidopsis thaliana under salt stress Tuba Acet1
•
Asım Kadıog˘lu2
Received: 14 April 2020 / Revised: 12 August 2020 / Accepted: 24 August 2020 / Published online: 29 August 2020 Ó Prof. H.S. Srivastava Foundation for Science and Society 2020
Abstract SOS5 locus, encodes cell wall adhesion protein under salt stress conditions in plants, and it is required for normal cell expansion as well as for sustaining cell wall integrity and structure. However, it is still unknown how this gene locus-ABA cross-talk and interacts with the antioxidant mechanism under salt stress conditions. For this purpose, the study focused on mutant sos5-1 plant treated with ABA under NaCl stress and observed its growth and development as well as stomatal aperture, lipid peroxidation, proline, hydrogen peroxide (H2O2) and ABA contents, and some antioxidant enzyme activities. In addition, the expression levels of ABA related genes have been analysed by RT-PCR after stress application. According to findings, sos5-1 mutant plants treated with ABA under salt stress resulted in eliminated cellular damage compared to those which are solely exposed to salt stress; other observations include closing of stomata, decreased H2O2 content, increased amount of proline, and similarity with the wild type due to induced antioxidant enzyme activities. Besides, both ABA biosynthetic and inducible gene expressions of the mutant plant under salt stress were lower compared to the control, and catabolism gene expression was higher. As a result, SOS5 gene in synergy with ABA, scavenge the ROS by stimulating antioxidant system, leads to an increase in stress related gene expressions and thus contributes to salinity tolerance.
& Tuba Acet [email protected] 1
Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Gu¨mu¨s¸ hane University, 29100 Gu¨mu¨s¸ hane, Turkey
2
Department of Biology, Faculty of Science, Karadeniz Technical University, 61080 Trabzon, Turkey
This study is significant in the way that it shows how SOS5 gene locus, under salt stress conditions, interacts with antioxidant system in sustaining cell wall integrity. Keywords Abscisic acid Antioxidant enzymes Arabidopsis thaliana Gene expression Salt stress sos51 mutant
Introduction Plants are sedentary organisms, and they are prone to many environmental stress factors throughout their lives. Salinity, especially NaCl, is one of the prominent stress factors limiting plant growth and development (Sun et al. 2020). With inaccurate agriculture and irrigation practices as well as industrial activities leading to salt effects on millions of hectares of land (FAO 2016), salinity becomes a global problem for humanity. The harmful effects of NaCl on plants result from decreased usage of water in soil due to sodium accumulation and toxicity of Na?/Cl- ions on plants. The plants named halophytes have developed special strategies in order to cope with salinity (F
Data Loading...
