A simplified approach to control cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-med
- PDF / 2,559,432 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 0 Downloads / 184 Views
TISSUE ENGINEERING CONSTRUCTS AND CELL SUBSTRATES Original Research
A simplified approach to control cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-mediated RGD linkage A. M. Porras Hernández
1
●
H. Pohlit1 F. Sjögren1 L. Shi2 D. Ossipov2,3 M. Antfolk4,5 M. Tenje1 ●
●
●
●
●
1234567890();,:
1234567890();,:
Received: 4 May 2020 / Accepted: 30 September 2020 © The Author(s) 2020
Abstract In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine–glycine–aspartate onto the hydrogel surface via radical mediated thiol–ene reaction using the nonreacted Am groups. We show that 10 × 10 µm2 squares could be patterned with sharp features and a good resolution. The smallest area that could be patterned resulting in good cell adhesion was 25 × 25 µm2 squares, showing single-cell adhesion. Mouse brain endothelial cells adhered and remained in culture for up to 7 days on 100 × 100 µm2 square patterns. We see potential for this material combination for future use in novel organ-on-chip models and tissue engineering where the location of the cells is of importance and to further study endothelial cell biology. Graphical Abstract
1 Introduction * M. Tenje [email protected] 1
Science for Life Laboratory, Department of Materials Science and Engineering, Uppsala University, Uppsala, Sweden
2
Department of Chemistry-Ångström, Uppsala University, Uppsala, Sweden
3
Department of Biosciences and Nutrition (BioNut), Karolinska Institutet, Huddinge, Sweden
4
BRIC—Biotech Research and Innovation Centre, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
5
Novo Nordisk Foundation Center for Stem Cell Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Biomaterials are widely used in biological research and pharmaceutical development as biomimetic cell culture scaffolds to increase the in vivo resemblance of in vitro models [1]. For this purpose, hydrogels belong to a particularly attractive group of biomaterials due to their highwater content, which mimics the in vivo extracellular matrix (ECM) mechanical and physical properties. Furthermore, their high permeability for oxygen and nutrients [2] is important to support long-term cell cultures. In most situations, cell adhesion on the whole hydrogel scaffold is preferred, but for some applications, such as cell–cell interaction studies, one may wish to control the cell adhesion spatially
Data Loading...
