Cell Culture Techniques
Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively
- PDF / 1,652,944 Bytes
- 25 Pages / 504 x 720 pts Page_size
- 9 Downloads / 213 Views
. Introduction The aim of Guidance on Good Cell Culture Practice (GCCP) is to promote the maintenance of high standards and to reduce uncertainty in the development and application of animal and human cell and tissue culture procedures and products by encouraging the greater international harmonisation, rationalisation and standardisation of in vitro laboratory practices; quality control systems; safety procedures; recording and reporting; and compliance with laws, regulations and ethical principles. Here, the basic concepts of GCCP principles will be discussed in order to demonstrate their
Michael Aschner et al. (eds.), Cell Culture Techniques, Neuromethods, vol. 56, DOI 10.1007/978-1-61779-077-5_1, © Springer Science+Business Media, LLC 2011
1
2
Bal-Price and Coecke
role in the development, optimisation and validation of in vitro test methods to ensure more robust (relatively insensitive to minor changes in the protocol) and easy transferable test methods. The GCCP document (a full version see (1)) provides the basis for an internationally agreed-upon and consistent scientific high quality approach for the development of new alternative cell- and tissuebased test methods (2) with potential regulatory applicability and is referenced in the OECD Advisory Document on The Application of the Principles of GLP to In Vitro Studies (3,4). This guidance is not only required to meet regulatory requirements for toxicity testing of chemicals and products of various kinds but also to serve the rapidly expanding use of in vitro systems in basic research as well. Furthermore, significant developments are certain to result in the use of in vitro systems for high-throughput screening in pharmacology and toxicology and systems biology approaches (5); the enlarging fields of genomics, proteomics and metabonomics; the field of stem cell development and standardisation; and the use of biomarkers of disease, susceptibility, exposure and effect.
2. The Principles of GCCP The GCCP sets the minimum standards for any in vitro work involving cell and tissue cultures. However, its detailed implementation depends on the nature of the work involved. Based on review by a broad range of experts and organisations, the aim of GCCP is to foster consensus among all concerned with the use of cell and tissue culture systems, in order to: – Establish and maintain best cell and tissue culture practice. – Promote effective quality control systems. – Facilitate education and training. – Assist journal editors and editorial boards. – Assist research funding bodies. – Facilitate the interpretation and application of conclusions based on in vitro work. The GCCP guidance is based upon the following six operational principles. 2.1. First GCCP Principle
Establishment and maintenance of a sufficient understanding of the in vitro system and of the relevant factors which could affect it.
Guidance on Good Cell Culture Practice (GCCP)
3
The essential elements for assuring reliable and accurate work when using in vitro cell- and tissue-based systems are
Data Loading...
