Antidepressant Activity of Flavonoids and Thick Extract from Filipendula ulmaria Fruit
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Pharmaceutical Chemistry Journal, Vol. 54, No. 8, November, 2020 (Russian Original Vol. 54, No. 8, August, 2020)
MEDICINAL PLANTS ANTIDEPRESSANT ACTIVITY OF FLAVONOIDS AND THICK EXTRACT FROM Filipendula ulmaria FRUIT V. A. Kurkin,1,* K. N. Sazanova,1 E. N. Zaitseva,1 S. Kh. Sharipova,1 O. E. Pravdivtseva,1 E. V. Avdeeva,1 E. P. Gladunova,1 and N. R. Varina1 Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 54, No. 8, pp. 10 – 12, August, 2020.
Original article submitted March 31, 1990. The flavonoids quercetin and spireoside (quercetin 4¢-O-b-D-glucopyranoside) were isolated for the first time from fruit of meadowsweet [Filipendula ulmaria (L.) Maxim.] and identified using UV spectrophotometry, NMR spectroscopy, mass spectrometry, and acid hydrolysis. The isolated flavonoids and the thick extract of meadowsweet fruit possessed pronounced antidepressant activity according to the Despair test. Keywords: meadowsweet, Filipendula ulmaria (L.) Maxim., fruit, thick extract, flavonoids, spireoside, quercetin, antidepressant activity.
Meadowsweet [Filipendula ulmaria (L.) Maxim., Rosaceae] herb and flowers are used in science-based and folk medicine [1 – 3]. The aerial part of this plant is rich in various biologically active compounds that are dominated by phenolic compounds (simple phenols, phenylpropanoids, flavonoids, tanning agents), saponins, and others [2, 3]. Nootropic, hepatoprotective, antioxidant, and anti-inflammatory properties were reported for extracts of flowers, leaves, and herb of F. ulmaria and flavonoids isolated from the raw material [3 – 7]. In our opinion, meadowsweet fruit are also promising medicinal plant raw material. Previously, diuretic activity was found by us for the thick extract of meadowsweet fruit. Only tanning agents and fatty oil (triglycerides of oleic, linoleic, and linolenic acids) were reported from fruit of this plant [2]. Therefore, studies of the chemical composition of meadowsweet fruit and its pharmacological properties are crucial. The goal of the present work was to determine the flavonoid composition of meadowsweet fruit and the antidepressant activity of flavonoids and the thick extract from it. 1 *
EXPERIMENTAL PART Meadowsweet fruit were collected in August 2018 in Samara Region (near Alekseevka, Kinel?skii District) and dried in air in the shade. Ground air-dried fruit (200.0 g) was extracted with EtOH (70%) in a 1:5 raw-material–extractant ratio first at room temperature (2´) for 24 h and then under reflux on a boiling-water bath for 30 min. The combined aqueous EtOH extract was evaporated under vacuum to 60 mL, mixed with silica gel L 40/100 (50 g), and dried. The dried powder (dry extract + silica gel) was placed on a layer of silica gel (8 cm in diameter, 5 cm in height) formed from a suspension in CHCl3. The chromatography column was eluted by CHCl3 and CHCl3–EtOH mixtures (99:1, 97:3, 95:5, 93:7, 90:10, 85:15, 80:20, 75:25, 70:30, 60:40). The separation of compounds was monitored by TLC on Sorbfil PTSKh-AF-A-UF plates using CHCl3–EtOH–H2O (26:16:3) and n
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