Green Synthesis of Silver Nanoparticles from Caralluma tuberculata Extract and its Antibacterial Activity
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Green Synthesis of Silver Nanoparticles from Caralluma tuberculata Extract and its Antibacterial Activity Zahra Zarei1 · Damoun Razmjoue2 · Javad Karimi3 Received: 25 March 2020 / Accepted: 9 May 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The plant extracts were applied for synthesis of Ag-nanoparticles and expected such biological processes benefit from advantages like eco-friendly, cost-effective, and safe for human use. Accordingly, polyphenol compounds and antioxidant properties of this understudy plant extract was initially qualified and quantified by GC-Mass and subsequently its ability for silver nanoparticle (AgNP) biosynthesis was examined. Consequently, under study (ethanol) extract was exposed to various content of A gNO3 solution over 0, 2.5, 5, 7.5, 10 and 15 mM for 24, 48, 72 and 96 h to search best operational conditions for AgNPs production, while sampling were done every 24 h monitoring initial absorption with UV–Vis spectroscopy. The peak with center at 460 nm emerged from surface plasmon resonance of AgNPs which is good indication of successful formation of AgNPs. The nanoparticles were characterized by DLS, SEM, TEM, XRD, and FTIR. Which reveal its spherical, shape and average diameter of 32 nm. Antioxidant activity tests showed that the half-maximal inhibitory concentration (IC50) in the C. tuberculata extract was 4.722 mg/ml. Antimicrobial activity of the AgNPs was tested by measurement of inhibition zone around discs modified with AgNPs in cultures of S. aureus, B. cereus, P. aeruginosa and E. coli. Keywords Green synthesis · Silver nanoparticles · Antibacterial activity · Antioxidant · Caralluma tuberculata
1 Introduction Nanoparticles at least over one of their dimension have size in the range of 1–100 nm. Amongst, this branch of materials owing to their high surface area and larger extent of surface reactive centers attain great attentions. Silver nanoparticles (AgNPs) have denote great potential in the medical field for treatments of infectious (bacterial, fungal, viral and parasitic), gastrointestinal tract, skin diseases (including acne), and some types of wounds and burns, which along its ability for action in oral rehydration solutions and disinfectants * Damoun Razmjoue [email protected] * Javad Karimi [email protected] 1
Department of Rangeland and Watershed Management, Faculty of Environment andNatural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran
2
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3
Department of Biology, Faculty of Sciences, Shiraz University, 71454 Shiraz, Iran
candidate this material for medicinal and biological purposes [1, 2]. Up to now, there are different methods used to synthesize various novel functional metallic nanostructures [3–8]. Biological organisms and materials such as microorganisms, biomass and plant extracts are alternative approaches for nanoparticle production owing to their lower harmful and toxicity to the e
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