Association with Leptin Gene c.-2548 G>A Polymorphism, Serum Leptin Levels, and Body Mass Index in Turkish Obese Pati
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ORIGINAL PAPER
Association with Leptin Gene c.-2548 G>A Polymorphism, Serum Leptin Levels, and Body Mass Index in Turkish Obese Patients Deniz Say Sahin • Cemil Tumer • Cemil Demir M. Murat Celik • Mustafa Celik • Edip Ucar • Ramazan Gunesacar
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Published online: 30 September 2012 Ó Springer Science+Business Media New York 2012
Abstract Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefore, in the present study, we aimed to investigate whether LEP c.-2548 G[A polymorphism was associated with serum leptin levels, lipid parameters, and body mass index in Turkish obese patients. Forty-seven obese patients and 48 healthy individuals were included in the study. Blood samples were collected for DNA extraction. LEP c.-2548 G[A polymorphism were detected using polymerase chain reaction–restriction fragment length polymorphism technique. Serum leptin levels and lipid parameters were measured by ELISA and enzyme colorimetric assay techniques, respectively. GA or AA genotypes and A allele carrier frequencies of the c.-2548 G[A polymorphism in the LEP were higher in obese (38.3, 34.0 and 72.3 %)
D. S. Sahin C. Demir Vocational Higher School of Health Services, Mardin Artuklu University, 47100 Mardin, Turkey C. Tumer Department of Physiology, Faculty of Medicine, Mustafa Kemal University, 31100 Hatay, Turkey M. M. Celik E. Ucar Department of Internal Medicine, Faculty of Medicine, Mustafa Kemal University, 31100 Hatay, Turkey M. Celik R. Gunesacar (&) Department of Medical Biology and Genetics, Faculty of Medicine, Kahramanmaras Sutcu Imam University, 46050 Kahramanmaras, Turkey e-mail: [email protected]
when compared with controls (14.6, 12.5, and 27.1 %; p = 0.011, 0.016, and 0.002, respectively). On the other hand, AA or AG genotypes were also related to increased serum leptin levels (p \ 0.001) and body mass index (p \ 0.0001). All these consequences showed that LEP 2548 AA or AG genotypes are important predictors for increased levels of leptin and BMI in Turkish obese patients and it may be a useful marker for obesity risk in our population. Keywords Leptin c.-2548 G[A Polymorphism Obesity Body mass index
Introduction Obesity is the most prevalent nutritional public health problem. The prevalence of obesity is increasing in most of westernized and developing countries in the world including Turkey [1, 2]. Obesity has a complex pathogenesis that results from interactions between genetic and environmental factors [3, 4]. In the medical literature, at least 37 obesity-related genes have been reported so far. Some of theme such as MC4R, POMC, FTO, and BDNF genes play a major role in the pathogenesis of obesity [5]. Leptin is a hormone that consists of 167-amino acid transcribed by the leptin gene (LEP) and secreted primarily by adipocytes [
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